Anti-Human TRPV2 (VRL1) (extracellular) Antibody | Alomone Labs ACC-139 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human TRPV2 (VRL1) (extracellular) Antibody

catalog :

ACC-139

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Hu J, Gao Y, Huang Q, Wang Y, Mo X, Wang P, et al. Flotillin-1 Interacts With and Sustains the Surface Levels of TRPV2 Channel. Front Cell Dev Biol. 2021;9:634160 pubmed publisher

image

image 1 :

Western blot analysis of human HMC3 microglial (lanes 1 and 5), Jurkat T-cell leukemia (lanes 2 and 6), HepG2 liver hepatoma (lanes 3 and 7) and chronic myelogenous K562 leukemia (lanes 4 and 8) cell lysates: - 1-4. Anti-Human TRPV2 (VRL1) (extracellular) Antibody (#ACC-139), (1:200).5-8. Anti-Human TRPV2 (VRL1) (extracellular) Antibody, preincubated with Human TRPV2/VRL1 (extracellular) Blocking Peptide (#BLP-CC139).

product information

CAT :

ACC-139

SKU :

ACC-139-CF_0.2 ml

Product Name :

Anti-Human TRPV2 (VRL1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Y5S1

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CC139

Homology :

Human only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KQAAPHLKAEVGNS, corresponding to amino acid residues 417-430 of human TRPV2

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-termini of all TRPs are intracellular3.According to IUPHAR, the TRP family is comprised of numerous subfamilies on the basis of sequence homology; TRPC, TRPM, TRPV, TRPA, TRPML, and TRPP1-4. The TRPV subfamily consists of six members, TRPV1-65.Four members of the TRPV family have been described as thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (52°C) 6,7. Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation8-10. TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway8. The TRPV2 channel is expressed in DRG neurons, different brain regions and non-neuronal tissues such as the spleen, lung and intestine and in components of the immune system5,11,12.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Strech-activated channel 2B, SAC2B

Short Description :

A Rabbit Polyclonal Antibody to TRPV2

Long Description :

Anti-Human TRPV2 (VRL1) (extracellular) Antibody (#ACC-139) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPV2 from human samples only. It will not recognize the protein from rat or mouse samples.

Negative Control :

BLP-CC139

Positive Control :

Synonyms :

Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Strech-activated channel 2B, SAC2B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV2

AB Specifictiy :

The antibody will not recognize the protein from rat or mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage