Anti-CaV1.2 (CACNA1C) (extracellular) Antibody | Alomone Labs ACC-130 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaV1.2 (CACNA1C) (extracellular) Antibody

catalog :

ACC-130

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes:

image 2 :

Western blot analysis of mouse TK-1 T-cell lymphoma cell line lysate (lanes 1 and 3) and mouse Neuro-2a neuroblastoma cell line lysate (lanes 2 and 4):

image 3 :

Western blot analysis of human Caco-2 colon carcinoma cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast carcinoma cell line lysate (lanes 2 and 4):

product information

CAT :

ACC-130

SKU :

ACC-130-CF_0.2 ml

Product Name :

Anti-CaV1.2 (CACNA1C) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q01815

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC130

Homology :

Mouse, human, rat - 15 out of 16 amino acids identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 16th loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KK(S)APESEPSNSTEGE, corresponding to amino acid residues 1429 – 1444 of mouse CaV1.2

Product Page - Scientific background :

All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium Voltage-Gated Channel Subunit Alpha1C

Short Description :

A Rabbit Polyclonal antibody to CaV1.2 (extracellular)

Long Description :

Anti-CaV1.2 (CACNA1C) (extracellular) Antibody (#ACC-130) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, and immunohistochemistry. It has been designed to recognize CaV1.2 from mouse, rat and human samples.

Negative Control :

BLP-CC130

Positive Control :

Synonyms :

Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium Voltage-Gated Channel Subunit Alpha1C

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1C

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage