Anti-TRPM7 (extracellular) Antibody | Alomone Labs ACC-129 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPM7 (extracellular) Antibody

catalog :

ACC-129

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human Raji B-cell lymphoma cell line lysate (lanes 2 and 6 ), mouse WEHI B-cell lymphoma cell line lysate (lanes 3 and 7) and human NK-92 natural killer cell line lysate (lanes 4 and 8): - 1-4. Anti-TRPM7 (extracellular) Antibody (#ACC-129), (1:200). 5-8. Anti-TRPM7 (extracellular) Antibody, preincubated with TRPM7 (extracellular) Blocking Peptide (BLP-CC129).

image 2 :

Expression of TRPM7 in rat parietal cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM7 (extracellular) Antibody (#ACC-129), (1:600), followed by goat anti-rabbit-AlexaFluor-488. A. TRPM7 immunoreactivity (green) appears in cortical neurons (arrows). B. Pre-incubation of the antibody with TRPM7 (extracellular) Blocking Peptide (BLP-CC129), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACC-129

SKU :

ACC-129-CF_0.2 ml

Product Name :

Anti-TRPM7 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q923J1

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC129

Homology :

Rat - identical, human - 13 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RFGAKWNYINAYDNH, corresponding to amino acid residues 942 - 956 of mouse TRPM7

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.1-4The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5TRPM7 and TRPM6 are involved in Mg2+ homeostasis and are unique among the TRP family members, possessing a functional kinase domain at their C-terminus.6,7 Although the kinase is not necessary to the function of the channel it may have a role in modulating the activation of channel 7.Recent work demonstrated that TRPM7 is a critical mediator of anoxic cell death.7,8

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily M member 7, Long transient receptor potential channel 7, LTRPC7, Channel-kinase 1, CHAK1, Transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK

Short Description :

A Rabbit Polyclonal antibody to TRPM7 (extracellular)

Long Description :

Anti-TRPM7 (extracellular) Antibody (#ACC-129) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize TRPM7 from rat, mouse and human samples.

Negative Control :

BLP-CC129

Positive Control :

Synonyms :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPM7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage