Anti-CACNG8 Antibody | Alomone Labs ACC-125 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNG8 Antibody

catalog :

ACC-125

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat testis (lanes 1 and 4), rat brain (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates: - 1-3. Anti-CACNG8 Antibody (#ACC-125), (1:400).4-6. Anti-CACNG8 Antibody, preincubated with CACNG8 Blocking Peptide (#BLP-CC125).

image 2 :

Expression of TARP γ8 (CACNG8) in rat hippocampus - Immunohistochemical staining of rat hippocampus using Anti-CACNG8 Antibody (#ACC-125). A. TARP γ8 staining (green) appears in neuronal soma (horizontal arrows) and in the neuronal processes (diagonal arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.

image 3 :

Cell surface detection ofSLC7A2 in live intact human THP-1 monocytic leukemia cells: - ___Cells.___Cells + rabbit IgG isotype control-FITC.___Cells + Anti-SLC7A2 (extracellular)-FITC Antibody(#ANT-103-F) (5 g).

product information

CAT :

ACC-125

SKU :

ACC-125-CF_0.2 ml

Product Name :

Anti-CACNG8 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8VHW5

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC125

Homology :

Rat, mouse, human - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ESLKRWNEERGLW(S)EK, corresponding to amino acid residues 2-17 of rat CACNG8

Product Page - Scientific background :

Transmembrane AMPA receptor regulatory proteins (TARPs) serve as auxiliary subunits of AMPA receptors that regulate functional aspects of these receptors such as: fast excitatory synaptic transmission, surface trafficking, enhancing synaptic clustering and increasing glutamate affinity. Generally, TARPs are responsible for regulating expression, channel properties and localization of AMPA receptors in the brain1.TARPs are non-pore-forming integral membrane proteins with four transmembrane domains that are widely expressed in the CNS. The TARP family is divided in Type I and type II subfamily. Type I TARPs comprise four calcium channel γ subunits: γ2, γ3, γ4, and γ-8, also known as Cacng2, 3, 4 and 8, respectively2,3.γ-8 TARP predominantly expressed in the telencephalon with abundant distribution on hippocampal excitatory synapses and extrasynaptic membranes. It is expressed in low levels in newborn and neonatal brain and can be found in higher levels in adult brain4. γ-8 plays an important role in increasing the number of synaptic and extrasynaptic AMPA receptors on dendrites and spines5. The C terminus of γ-8 contains 3 unique stretches of amino acids that are not found in any of the other TARPs. In addition, it contains PDZ-binding motifs2. In heterologous cells, γ-8 prolong the current rise time in response to small amounts of glutamate and slow GluA1 receptor desensitization and deactivation3.Several studies have shown that overexpression of TARP γ-8 increases the number of AMPARs in the plasma membrane. In γ-8 knockout mice, AMPA receptor expression is severely reduced from the synaptic and extrasynaptic membranes of dendrites and does not progress through the secretory pathway5,6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

CaVγ8, Voltage-dependent calcium channel gamma-8 subunit, TARP gamma-8, Transmembrane AMPAR regulatory protein gamma-8

Short Description :

A Rabbit Polyclonal Antibody to CACNG8 (CaV?8) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat TARP gamma-8 (CaVγ8, CACNG8). Anti-CACNG8 Antibody (#ACC-125) can be used in western blot and immunohistochemistry applications. It has been designed to recognize TARP gamma-8 from rat, mouse and human samples.

Negative Control :

BLP-CC125

Positive Control :

Synonyms :

CaVγ8, Voltage-dependent calcium channel gamma-8 subunit, TARP gamma-8, Transmembrane AMPAR regulatory protein gamma-8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNG8

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage