Anti-TRPV4 (extracellular) Antibody | Alomone Labs ACC-124 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPV4 (extracellular) Antibody

catalog :

ACC-124

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 10

Reference
Willard V, Leddy H, Palmer D, Wu C, Liedtke W, Guilak F. Transient receptor potential vanilloid 4 as a regulator of induced pluripotent stem cell chondrogenesis. Stem Cells. 2021;39:1447-1456 pubmed publisher
Woods S, Humphreys P, Bates N, Richardson S, Kuba S, Brooks I, et al. Regulation of TGFβ Signalling by TRPV4 in Chondrocytes. Cells. 2021;10: pubmed publisher
De Logu F, Trevisan G, Marone I, Coppi E, Padilha Dalenogare D, Titiz M, et al. Oxidative stress mediates thalidomide-induced pain by targeting peripheral TRPA1 and central TRPV4. BMC Biol. 2020;18:197 pubmed publisher
Espadas xc1 lvarez H, Mart xed nez Rend xf3 n J, Larre I, Matamoros Volante A, Romero Garc xed a T, Rosenbaum T, et al. TRPV4 activity regulates nuclear Ca2+ and transcriptional functions of β-catenin in a renal epithelial cell model. J Cell Physiol. 2021;236:3599-3614 pubmed publisher
Scheraga R, Abraham S, Grove L, Southern B, Crish J, Perelas A, et al. TRPV4 Protects the Lung from Bacterial Pneumonia via MAPK Molecular Pathway Switching. J Immunol. 2020;204:1310-1321 pubmed publisher
Cussac L, Cardouat G, Tiruchellvam Pillai N, Campagnac M, Robillard P, Montillaud A, et al. TRPV4 channel mediates adventitial fibroblast activation and adventitial remodeling in pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 2020;318:L135-L146 pubmed publisher
Naert R, Lopez Requena A, Voets T, Talavera K, Alpizar Y. Expression and Functional Role of TRPV4 in Bone Marrow-Derived CD11c+ Cells. Int J Mol Sci. 2019;20: pubmed publisher
Onishi M, Yamanaka K, Miyamoto Y, Waki H, Gouraud S. Trpv4 involvement in the sex differences in blood pressure regulation in spontaneously hypertensive rats. Physiol Genomics. 2018;50:272-286 pubmed publisher
Caires R, Sierra Valdez F, Millet J, Herwig J, Roan E, Vásquez V, et al. Omega-3 Fatty Acids Modulate TRPV4 Function through Plasma Membrane Remodeling. Cell Rep. 2017;21:246-258 pubmed publisher
Shavit Stein E, Artan Furman A, Feingold E, Ben Shimon M, Itzekson Hayosh Z, Chapman J, et al. Protease Activated Receptor 2 (PAR2) Induces Long-Term Depression in the Hippocampus through Transient Receptor Potential Vanilloid 4 (TRPV4). Front Mol Neurosci. 2017;10:42 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:200).2. Anti-TRPV4 (extracellular) Antibody, preincubated with TRPV4 (extracellular) Blocking Peptide (#BLP-CC124).

image 2 :

Expression of TRPV4 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 (extracellular) Antibody (# ACC-124). A. TRPV4 labeling appears in neuronal cell bodies. B. Nuclear staining using DAPI as counter stain. C. merged images of A and B.

image 3 :

Expression of TRPV4 in human U-87 cells - Cell surface detection ofTRPV4 in live intact human brain glioblastoma U-87 cells. A. Extracellular staining of cells with Anti-TRPV4 (extracellular)Antibody (#ACC-124) (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merged images of live cells with A.

product information

CAT :

ACC-124

SKU :

ACC-124-CF_0.2 ml

Product Name :

Anti-TRPV4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9ERZ8

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC124

Homology :

Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

OTRPC4, VRL-2, VR-OAC, TRP12

Short Description :

A Rabbit Polyclonal Antibody to TRPV4 Channel

Long Description :

Anti-TRPV4 (extracellular) Antibody (#ACC-124) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples.

Negative Control :

BLP-CC124

Positive Control :

Synonyms :

OTRPC4, VRL-2, VR-OAC, TRP12

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage