Anti-TRPV4 (extracellular)-FITC Antibody | Alomone Labs ACC-124-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPV4 (extracellular)-FITC Antibody

catalog :

ACC-124-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of TRPV4 in live intact mouse J774 macrophage cells: Cell surface detection of TRPV4 in live intact mouse J774 macrophage cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-TRPV4 (extracellular)-FITC Antibody (#ACC-124-F), (2.5 µg).

product information

CAT :

ACC-124-F

SKU :

ACC-124-F_15 mcl

Product Name :

Anti-TRPV4 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9ERZ8

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC124

Homology :

Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

OTRPC4, VRL-2, VR-OAC, TRP12, Osm-9-Like TRP Channel 4

Short Description :

A Rabbit Polyclonal Antibody to TRPV4 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-TRPV4 (extracellular) Antibody (#ACC-124) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples. Anti-TRPV4 (extracellular)-FITC Antibody (#ACC-124-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

OTRPC4, VRL-2, VR-OAC, TRP12, Osm-9-Like TRP Channel 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-124), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage