Anti-TRPC6 (extracellular) Antibody | Alomone Labs ACC-120 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPC6 (extracellular) Antibody

catalog :

ACC-120

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig s1a	Kim J, Hwang K, Dang B, Eom M, Kong I, Gwack Y, et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 2021;12:6537 pubmed publisher
western blot; human; loading ...; fig 1b	Diez Bello R, Jardin I, Salido G, Rosado J. Orai1 and Orai2 mediate store-operated calcium entry that regulates HL60 cell migration and FAK phosphorylation. Biochim Biophys Acta Mol Cell Res. 2017;1864:1064-1070 pubmed publisher
	Matthews A, Soni H, Robinson Freeman K, John T, Buddington R, Adebiyi A. Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species. Int J Mol Sci. 2021;22: pubmed publisher
	Bocchero U, Falleroni F, Mortal S, Li Y, Cojoc D, LAMB T, et al. Mechanosensitivity is an essential component of phototransduction in vertebrate rods. PLoS Biol. 2020;18:e3000750 pubmed publisher
	Ortiz Muñoz G, Yu M, Lefrançais E, Mallavia B, Valet C, Tian J, et al. Cystic fibrosis transmembrane conductance regulator dysfunction in platelets drives lung hyperinflammation. J Clin Invest. 2020;130:2041-2053 pubmed publisher
	Sadowska A, Hitzl W, Karol A, Jaszczuk P, Cherif H, Haglund L, et al. Differential regulation of TRP channel gene and protein expression by intervertebral disc degeneration and back pain. Sci Rep. 2019;9:18889 pubmed publisher
	Christensen S, Johansson S, Radziwon Balicka A, Warfvinge K, Haanes K, Edvinsson L. MEK1/2 inhibitor U0126, but not nimodipine, reduces upregulation of cerebrovascular contractile receptors after subarachnoid haemorrhage in rats. PLoS ONE. 2019;14:e0215398 pubmed publisher
	Delcroix V, Mauduit O, Tessier N, Montillaud A, Lesluyes T, Ducret T, et al. The Role of the Anti-Aging Protein Klotho in IGF-1 Signaling and Reticular Calcium Leak: Impact on the Chemosensitivity of Dedifferentiated Liposarcomas. Cancers (Basel). 2018;10: pubmed publisher
	Wang Z, Yang K, Zheng Q, Zhang C, Tang H, Babicheva A, et al. Divergent changes of p53 in pulmonary arterial endothelial and smooth muscle cells involved in the development of pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 2019;316:L216-L228 pubmed publisher
	Diez Bello R, Jardin I, Lopez J, El Haouari M, Ortega Vidal J, Altarejos J, et al. (-)‑Oleocanthal inhibits proliferation and migration by modulating Ca2+ entry through TRPC6 in breast cancer cells. Biochim Biophys Acta Mol Cell Res. 2019;1866:474-485 pubmed publisher
	Jardin I, Diez Bello R, Lopez J, Redondo P, Salido G, Smani T, et al. TRPC6 Channels Are Required for Proliferation, Migration and Invasion of Breast Cancer Cell Lines by Modulation of Orai1 and Orai3 Surface Exposure. Cancers (Basel). 2018;10: pubmed publisher
	L xf3 pez E, G xf3 mez Gordo L, Cantonero C, Bermejo N, P xe9 rez G xf3 mez J, Granados M, et al. Stanniocalcin 2 Regulates Non-capacitative Ca2+ Entry and Aggregation in Mouse Platelets. Front Physiol. 2018;9:266 pubmed publisher
	He X, Song S, Ayon R, Balisterieri A, Black S, Makino A, et al. Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells. Am J Physiol Cell Physiol. 2018;314:C504-C517 pubmed publisher
	Oishi S, Suzuki N, Hasui Y, Homma T, Obana M, Nagayama T, et al. Sustained Activation of Guanylate Cyclase-A with TDT, a Natriuretic Peptide Derivative, Exhibits Cardiorenal Protection in Dahl Salt-Sensitive Hypertensive Rats. J Pharmacol Exp Ther. 2017;363:402-410 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysates: - 1. Anti-TRPC6 (extracellular) Antibody (#ACC-120), (1:200).2. Anti-TRPC6 (extracellular) Antibody, preincubated with TRPC6 (extracellular) Blocking Peptide (#BLP-CC120).

image 2 :

Expression of TRPC6 in rat PC12 cells - Cell surface detection ofTRPC6 in intact living PC12 cells. A. Extracellular staining of cells with Anti-TRPC6 (extracellular)Antibody (#ACC-120) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining with DAPI as the counterstain. C. Merged image of A and B.

image 3 :

TRPC6 is required for stretch activation in myocytes. - Electrophysiological recordings of myocytes incubated with Anti-TRPC6 (extracellular) Antibody (#ACC-120) in the absence (upper left) and in the presence (lower left) of the control peptide antigen. Immunocytochemical staining of living rat cerebral artery smooth muscle cells using Anti-TRPC6 (extracellular) antibody. Preincubation with the control peptide antigen blocks TRPC6 immunostaining (lower right panel).Adapted from Gonzales A.L. et al. with permission of the American Association for the Advancement of Science.

product information

CAT :

ACC-120

SKU :

ACC-120-CF_0.2 ml

Product Name :

Anti-TRPC6 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61143

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC120

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DNVKYYNLARIKWD, corresponding to amino acid residues 573-586 of rat TRPC6

Product Page - Scientific background :

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels1-4.The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: TRPC1, TRPC4, TRPC5 TRPC3, TRPC6, TRPC7 and TRPC22,5. They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC6 can form heterotetramers with TRPC3 and TRPC7. It is primarily expressed in brain, lung and muscle. High levels of expression of the channel were also found in human platelets. Recently it was reported that TRPC6 is also expressed in the kidney where a mutated channel has been implicated in kidney failure disease6,7.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Short transient receptor potential channel 6, Transient receptor protein 6, TRP6

Short Description :

A Rabbit Polyclonal Antibody to TRPC6 Channel

Long Description :

Anti-TRPC6 (extracellular) Antibody (#ACC-120) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize TRPC6 from rat, mouse and human samples.

Negative Control :

BLP-CC120

Positive Control :

Synonyms :

Short transient receptor potential channel 6, Transient receptor protein 6, TRP6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage