Anti-TRPC1 (extracellular) Antibody | Alomone Labs ACC-118 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPC1 (extracellular) Antibody

catalog :

ACC-118

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Anand P, Filipenko P, Huaman J, Lyudmer M, Hossain M, Santamaría C, et al. Selective Inhibition of Liver Cancer Cells Using Venom Peptide. Mar Drugs. 2019;17: pubmed publisher
Yap J, Tai Y, Fröhlich J, Fong C, Yin J, Foo Z, et al. Ambient and supplemental magnetic fields promote myogenesis via a TRPC1-mitochondrial axis: evidence of a magnetic mitohormetic mechanism. FASEB J. 2019;33:12853-12872 pubmed publisher
Wang Z, Yang K, Zheng Q, Zhang C, Tang H, Babicheva A, et al. Divergent changes of p53 in pulmonary arterial endothelial and smooth muscle cells involved in the development of pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 2019;316:L216-L228 pubmed publisher
Gonz lez S nchez P, del Arco A, Esteban J, Satr stegui J. Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons. Front Cell Neurosci. 2017;11:363 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-TRPC1 (extracellular) Antibody (#ACC-118), (1:200).2. Anti-TRPC1 (extracellular) Antibody, preincubated with TRPC1 (extracellular) Blocking Peptide (#BLP-CC118).

image 2 :

Peptide (C)SMGQMLQDFGK corresponding to amino acid residues 495-505 of rat TRPC1 (AccessionQ9QX01). 2ndextracellular loop.

image 3 :

Expression of TRPC1 in PC12 cells - Cell surface detection ofTRPC1 in intact living PC12 cells. A. Extracellular staining of cells withAnti-TRPC1 (extracellular) Antibody (#ACC-118) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining with DAPI as the counterstain. C. Merged images of A and B.

product information

CAT :

ACC-118

SKU :

ACC-118-CF_0.2 ml

Product Name :

Anti-TRPC1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QX01

Applications :

IC IF IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC118

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SMGQMLQDFGK, corresponding to amino acid residues 495-505 of rat TRPC1

Product Page - Scientific background :

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels1-4.The TRPC subfamily consists of seven proteins named TRPC1 to 7, which can be further divided into four subgroups based on their sequence homology and functional similarities: 1.) TRPC1 2.) TRPC4 and TRPC5 3.) TRPC3, TRPC6, TRPC7 4.) TRPC22,5. They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC1 was the first mammalian TRP protein that was reported to form an ion channel2. It can co-assemble with other TRPC subunits (TRPC3, TRPC4, TRPC5) to form heterotetramers whose properties are distinct from that of their homomeric form. The existence of the TRPC1 homomers has not been established as yet1-3.The TRPC1, TRPC4 and TRPC5 can be activated either by Ca2+, store depletion or by GPCR stimulation pathways, while TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs. TRPC1, 4 and TRPC5 are assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells6.

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

Short transient receptor potential channel 1, Transient receptor protein 1, TRP1

Short Description :

A Rabbit Polyclonal Antibody to TRPC1 Channel

Long Description :

Anti-TRPC1 (extracellular) Antibody (#ACC-118) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging, and immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize TRPC1 from mouse, rat, and human samples.

Negative Control :

BLP-CC118

Positive Control :

Synonyms :

Short transient receptor potential channel 1, Transient receptor protein 1, TRP1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage