antibody

Anti-IP3 Receptor-2 (ITPR2)-ATTO Fluor-594 Antibody

ACC-116-AR

polyclonal

domestic rabbit

ATTO 594

NA

mouse, rat

immunohistochemistry, immunocytochemistry

ACC-116-AR_50 mcl

Anti-IP3 Receptor-2 (ITPR2)-ATTO Fluor-594 Antibody

Antibodies

Antibodies

Polyclonal

P29995

IF IHC

Mouse

Rabbit

BLP-CC116

Mouse, human - 13/14 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Intracellular, C-terminus

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)RLGFLGSNTPHENH, corresponding to amino acid residues 2683-2696 of rat IP3R2

Inositol 1,4,5-trisphosphate receptors (IP3R) are intracellular Ca2+ release channels located on the endoplasmic reticulum (ER) and mediate Ca2+ mobilization from the ER to the cytoplasm in response to the binding of the second messenger, inositol 1,4,5-trisphosphate (IP3)1. IP3-induced Ca2+ release is triggered by various external stimuli, and most non-excitable cells use this mechanism as the primary Ca2+ signaling pathway. IP3Rs are therefore thought to have important physiological roles in various cell types and tissues2.Three subtypes of IP3Rs, derived from three distinct genes, have been identified in mammals3. All three receptors have six transmembrane domains and a pore region between TM5 and TM6. The N-terminus as well as the C-terminus are cytoplasmic. Each IP3R consists of an N-terminal ligand binding domain (LBD) and a C-terminal domain which is linked by a long regulatory domain. The C-terminus is constitutively active, suggesting that the regulatory domain is required to maintain the suppression of channel activity4. Type 2 IP3R (IP3R2) is expressed in various tissues and cell lines. IP3R2 mRNA is localized in the intralobular duct cells of the submandibular gland, the urinary tubule cells of the kidney, the epithelial cells of epididymal ducts and the follicular granulosa cells of the ovary5. IP3R2 is active during osteoclast differentiation, and its absence causes a partial defect in osteoclastic differentiation.

IF IHC

Alomone Labs

Inositol 1,4,5-trisphosphate receptor type 2, IP3R2, InsP3R2, IP3 receptor isoform 2

A Rabbit Polyclonal Antibody to IP3 Receptor-2 Conjugated to the Fluorescent Dye ATTO-594

Anti-IP3 Receptor-2 (ITPR2) Antibody (#ACC-116) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IP3R2 from rat, mouse and human samples. Anti-IP3 Receptor-2 (ITPR2)-ATTO Fluor-594 Antibody (#ACC-116-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-IP3 Receptor-2 (ITPR2)-ATTO Fluor-594 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

BLP-CC116

NA

Inositol 1,4,5-trisphosphate receptor type 2, IP3R2, InsP3R2, IP3 receptor isoform 2

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ITPR2

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #ACC-116), and immunohistochemistry (labeled antibody).

1 µg peptide per 1 µg antibody

1:60

Contact Alomone for information