Anti-CACNG5 (extracellular) Antibody | Alomone Labs ACC-115 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNG5 (extracellular) Antibody

catalog :

ACC-115

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-CACNG5 (extracellular) Antibody (#ACC-115), (1:1000).3,4. Anti-CACNG5 (extracellular) Antibody, preincubated with CACNG5 (extracellular) Blocking Peptide (#BLP-CC115).

image 2 :

Peptide (C)SESTVNVLKMIRS corresponding to amino acid residues 87-99 of rat CACNG5 (AccessionQ8VHW8). 1stextracellular loop.

image 3 :

Expression of CaV?5 (CACNG5) in living rat PC12 cells - Cell surface detection ofCaV?5 (CACNG5) in intact living rat PC12 cells usingAnti-CACNG5(extracellular) Antibody(#ACC-115) (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Extracellular staining (red) merged with live view of the cells. B. Extracellular staining merged with nuclear staining using DAPI as the counterstain (blue).

product information

CAT :

ACC-115

SKU :

ACC-115-CF_0.2 ml

Product Name :

Anti-CACNG5 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8VHW8

Applications :

IC IF LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC115

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SESTVNVLKMIRS, corresponding to amino acid residues 87-99 of rat CACNG5

Product Page - Scientific background :

Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. On the basis of their voltage activation properties, the voltage-gated Ca2+ channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels.2. CaV channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3.CaVγ subunits inhibit CaV channel activity and modulate its activation and inactivation kinetics. CaVγ subunits have little effect on CaV channel trafficking4. The γ subunit is an integral membrane protein. The γ subunit family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues5.The CaVγ5 subunit is highly expressed in the liver, kidney, heart, lung, skeletal muscles, and, with a lower abundance, in testes5. The close association of epilepsy and ataxia with mutations in other neuronal voltage-dependent Ca2+ channels suggests these are potential candidate phenotypes for defects in the CaVγ5 (CACNG5) gene6.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

CaVγ5, Voltage-dependent calcium channel gamma-5 subunit, TARP gamma-5, Transmembrane AMPAR regulatory protein gamma-5

Short Description :

A Rabbit Polyclonal Antibody to CACNG5 (CaVγ5) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat CaVγ5. Anti-CACNG5 (extracellular) Antibody (#ACC-115) can be used in western blot and live cell imaging experiments. It has been designed to recognize CaVγ5 from rat, mouse and human samples.

Negative Control :

BLP-CC115

Positive Control :

Synonyms :

CaVγ5, Voltage-dependent calcium channel gamma-5 subunit, TARP gamma-5, Transmembrane AMPAR regulatory protein gamma-5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNG5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage