Anti-CACNG6 (extracellular) Antibody | Alomone Labs ACC-112 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNG6 (extracellular) Antibody

catalog :

ACC-112

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat skeletal muscle lysate: - 1. Anti-CACNG6 (extracellular) Antibody (#ACC-112), (1:200).2. Anti-CACNG6 (extracellular) Antibody, preincubated with CACNG6 (extracellular) Blocking Peptide (#BLP-CC112).

image 2 :

Expression of CaVγ6 (CACNG6) in rat brain - Immunohistochemical staining of rat cingulate cortex using Anti-CACNG6 (extracellular) Antibody (#ACC-112), (1:400). A. CaVγ6 staining (green) appears in the pyramidal layer (arrow). B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.

product information

CAT :

ACC-112

SKU :

ACC-112-CF_0.2 ml

Product Name :

Anti-CACNG6 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8VHW3

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC112

Homology :

Mouse - identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CIKRLWQADVPAGR, corresponding to amino acid residues 87-100 of rat CACNG6

Product Page - Scientific background :

The calcium channel voltage-dependent gamma subunit 6 encoded by the CACNG6 gene, encodes an integral membrane protein. Its role is to stabilize the calcium channel during the channels inactive state. This subunit is responsible for decreasing Ca2+ currents when it is co-expressed with the low voltage activated Ca2+ channel subunit1,2. The calcium channel γ subunits comprise eight members that share a common topology. This family is divided in three clusters: The first- γ1 and γ6, second γ5 and γ7 and third γ2,3,4,8. CaVγ6 protein is predominantly expressed in striated muscle cells. It is also expressed in skeletal and cardiac muscles and slightly in the brain as a separate isoform.CaVγ6 structure includes four transmembrane domains (TM) with short intracellular N- and C-terminus. The first TM with a specifically motif- GxxxA is critical for CaVγ6 inhibitory function2. Phylogenetic analysis has suggested that all γ subunits developed from a single ancestral gene through tandem repeat and chromosome duplication3.Voltage-dependent calcium channels are important regulators of calcium influx and are important for several physiological processes including- synaptic transmission, muscle contraction, neurogenesis, hormone secretion, cell motility, cell division and development.Several recent studies have shown a negative correlation of CaVγ6 gene expression to chronic obstructive pulmonary disease, responses of the human airway epithelium following injury and parenchyma in lung tissues1,3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

CaVγ6, Voltage-dependent calcium channel gamma-6 subunit

Short Description :

A Rabbit Polyclonal Antibody to CACNG6 (CaVγ6) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CaVγ6. Anti-CACNG6 (extracellular) Antibody (#ACC-112) can be used in western blot and immunohistochemistry. The antibody recognizes an extracellular epitope and is thus ideal for detecting CaVγ6 in living cells. It has been designed to recognize CaVγ6 from rat, mouse and human samples.

Negative Control :

BLP-CC112

Positive Control :

Synonyms :

CaVγ6, Voltage-dependent calcium channel gamma-6 subunit

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNG6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage