Anti-CACNG1 (extracellular) Antibody | Alomone Labs ACC-111 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNG1 (extracellular) Antibody

catalog :

ACC-111

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat skeletal muscle lysate: - 1. Anti-CACNG1 (extracellular) Antibody (#ACC-111), (1:400).2. Anti-CACNG1 (extracellular) Antibody, preincubated with CACNG1 (extracellular) Blocking Peptide (#BLP-CC111).

image 2 :

Expression of CaVγ1 (CACNG1) in rat skeletal muscle - Immunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-CACNG1 (extracellular) Antibody (#ACC-111), (1:100), followed by DAB. CaVγ1 staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow). Hematoxilin is used as the counterstain.

image 3 :

Western blot analysis of rat skeletal muscle lysate: - 1.Anti-CACNG1(extracellular) Antibody(#ACC-111) (1:400).2. Anti-CACNG1(extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-111

SKU :

ACC-111-CF_0.2 ml

Product Name :

Anti-CACNG1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97707

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC111

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DHWAVLSPHLEHHNE, corresponding to amino acid residues 30-44 of rat CACNG1

Product Page - Scientific background :

Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+conducting pores in the plasma membrane1. On the basis of their voltage activation properties, the voltage-gated Ca2+ channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels.2. CaV channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3.CaVγ subunits inhibit CaV channel activity and modulate its activation and inactivation kinetics. CaVγ subunits have little effect on CaV channel trafficking4. The γ subunit is an integral membrane protein. The γ subunit family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues5.CaVγ1 is solely expressed in skeletal muscle and associates with the α1S (CaV1.1) subunit6.Knockout mice are indeed viable and show no extreme phenotype. It seems that γ1 enhances the inactivation of ER Ca2+ release in a voltage-dependent manner6.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

CaVγ1, CACNLG, Voltage-dependent calcium channel gamma-1 subunit, Dihydropyridine-sensitive L-type, skeletal muscle Ca2+ channel subunit gamma

Short Description :

A Rabbit Polyclonal Antibody to CACNG1 (CaVγ1) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CaVγ1. Anti-CACNG1 (extracellular) Antibody (#ACC-111) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize CaVγ1 from rat, mouse and human samples.

Negative Control :

BLP-CC111

Positive Control :

Synonyms :

CaVγ1, CACNLG, Voltage-dependent calcium channel gamma-1 subunit, Dihydropyridine-sensitive L-type, skeletal muscle Ca2+ channel subunit gamma

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNG1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage