Anti-CACNG7 (extracellular) Antibody | Alomone Labs ACC-109 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNG7 (extracellular) Antibody

catalog :

ACC-109

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-CACNG7 (extracellular) Antibody (#ACC-109), (1:1000).3,4. Anti-CACNG7 (extracellular) Antibody, preincubated with CACNG7 (extracellular) Blocking Peptide (#BLP-CC109).

product information

CAT :

ACC-109

SKU :

ACC-109-CF_0.2 ml

Product Name :

Anti-CACNG7 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P62955

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC109

Homology :

Rat, mouse -13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)REKGR(S)VASEYFLE, corresponding to amino acid residues 67-80 of human CACNG7

Product Page - Scientific background :

Transmembrane AMPA receptor regulatory proteins (TARPs) serve as auxiliary subunits of AMPA receptors and regulate functional aspects of these receptors such as: fast excitatory synaptic transmission, surface trafficking, synaptic clustering and glutamate affinity. Generally, TARPs are responsible for regulating expression, channel properties and localization of AMPA receptors in the brain1.TARPs are non-pore-forming integral membrane proteins with four transmembrane domains that are widely expressed in the CNS. TARP family is divided in two: Type I and type II. Type I TARPs include two calcium channel γ subunits: γ5 and γ7, also known as Cacng5 and Cacng7, respectively.They do not traffic AMPAR, they only modulate existing AMPAR channel function2.CaVγ7 (Transmembrane AMPA Receptor Regulatory Protein Isoform γ7) is highly expressed in Purkinje cells of the cerebellum and in glomerular synapses in the granule cell layer of the cerebellum and significantly enhances glutamate-evoked currents and modulates gating of AMPARs. CaVγ7 appears to derive from cerebellar Golgi cells2,3. CaVγ7 shows the largest sequence divergence compared to other TARP members. Its structure demonstrates a short C-terminus with an absence of the TTPV motif that is critical in the binding of TARP-associated AMPARs to PSD-95 and in clustering at the synapse3.Several recent studies have suggested that CaVγ7 has the ability to associate with extrasynaptic calcium-permeable AMPA receptors and plays an important role in regulating the presence of neuronal calcium-permeable AMPA receptors at synapses3. In addition, transfection of TARP CaVγ7 causes AMPA receptors to resensitize upon continued glutamate application3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

CaVγ7, Voltage-dependent calcium channel gamma-7 subunit, TARP gamma-7, Transmembrane AMPAR regulatory protein gamma-7

Short Description :

A Rabbit Polyclonal Antibody to CACNG7 (CaVγ7) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human CaVγ7. Anti-CACNG7 (extracellular) Antibody (#ACC-109) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting CaVγ7 in living cells. It has been designed to recognize CaVγ7 from rat, mouse and human samples.

Negative Control :

BLP-CC109

Positive Control :

Synonyms :

CaVγ7, Voltage-dependent calcium channel gamma-7 subunit, TARP gamma-7, Transmembrane AMPAR regulatory protein gamma-7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNG7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage