Anti-CACNB1 Antibody | Alomone Labs ACC-106 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNB1 Antibody

catalog :

ACC-106

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat cortex (lane 3 and 6): - 1-3. Anti-CACNB1 Antibody (#ACC-106), (1:800).4-6. Anti-CACNB1 Antibody, preincubated with CACNB1 Blocking Peptide (#BLP-CC106).

image 2 :

Expression of CACNB1 (CaVβ1) in rat hippocampus - Immunohistochemical staining of rat hippocampus using Anti-CACNB1 Antibody (#ACC-106). A. CaVβ1 staining (red) appears in dendrites of the stratum radiatum (arrow). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge image of A and B.

image 3 :

Peptide (C)KISNEHSPKLQIRSH corresponding to amino acid residues 397-411 of rat DLGAP1 (Accession P97836). Intracellular.

product information

CAT :

ACC-106

SKU :

ACC-106-CF_0.2 ml

Product Name :

Anti-CACNB1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P54283

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC106

Homology :

Mouse - identical; human - 14/16

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, adjacent to the C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DRATGEHASVHEYPGE, corresponding to amino acid residues 456-471 of rat CACNB1

Product Page - Scientific background :

Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. Based on their electrophysiological and pharmacological properties, CaV channels have traditionally been classified into L, T, N, P, Q, and R types2. L-type Ca2+ channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the subunits α2δ, γ, and β3. CaVβ subunits play critical roles in membrane trafficking of the channel complex and regulation of voltage-dependent gating. The CaVβ subunit binds to the endoplasmic reticulum (ER) retention signal in the I-II loop of the α1-subunit, which allows channels to traffic to the surface membrane4. Furthermore, CaVβ subunits not only allow for membrane trafficking of the channel complex, they also can play a role in determining the subcellular localization of channels on the surface membrane5. There are four distinct CaVβ subunits CaVβ1, CaVβ2, CaVβ3 and CaVβ46.Three splice variants exist for the β1 subunit: β1a, β1b and β1c. β1a is known to be expressed in skeletal muscle and brain, but not in smooth muscle or heart. β1a appears to be important for the functional expression of the α1 subunit in skeletal muscle7. β1b was identified by cloning in rat brain, heart and hippocampus, and differs from β1a by having a deletion of ~50 amino acids at residue 209, and having a 120-residue C-terminal elongation. β1c was cloned from human heart and hippocampus and has the same deletion as β1b, but lacks the C-terminal extension.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

CaVβ1, CAB1, CACNLB1, Voltage-dependent L-type calcium channel subunit beta-1

Short Description :

A Rabbit Polyclonal Antibody to CACNB1 (CaVβ1) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CaVβ1. Anti-CACNB1 Antibody (#ACC-106) can be used in western blot and immunohistochemistry applications. It has been designed to recognize CaVβ1 from rat, mouse and human samples.

Negative Control :

BLP-CC106

Positive Control :

Synonyms :

CaVβ1, CAB1, CACNLB1, Voltage-dependent L-type calcium channel subunit beta-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage