Anti-CACNB2 Antibody | Alomone Labs ACC-105 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNB2 Antibody

catalog :

ACC-105

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Al Katat A, Zhao J, Calderone A, Parent L. Sympathetic Stimulation Upregulates the Ca2+ Channel Subunit, CaVα2δ1, via the β1 and ERK 1/2 Pathway in Neonatal Ventricular Cardiomyocytes. Cells. 2022;11: pubmed publisher
Ednie A, Bennett E. Intracellular O-linked glycosylation directly regulates cardiomyocyte L-type Ca2+ channel activity and excitation-contraction coupling. Basic Res Cardiol. 2020;115:59 pubmed publisher
Morgenstern T, Park J, Fan Q, Colecraft H. A potent voltage-gated calcium channel inhibitor engineered from a nanobody targeted to auxiliary CaVβ subunits. elife. 2019;8: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-CACNB2 Antibody (#ACC-105), (1:800).3,4. Anti-CACNB2 Antibody, preincubated with CACNB2 Blocking Peptide (#BLP-CC105).

product information

CAT :

ACC-105

SKU :

ACC-105-CF_0.2 ml

Product Name :

Anti-CACNB2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8VGC3

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC105

Homology :

Mouse - identical; human - 11/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HEHVDRYVPHREHNHRE, corresponding to amino acid residues 571-587 of rat CACNB2

Product Page - Scientific background :

Voltage-dependent Ca2+ channels are a family of membrane proteins that allow cells to couple electrical activity to intracellular Ca2+ signaling1. Voltage-gated Ca2+ channels are classified as T, L, N, P, Q and R, and are distinguished by their sensitivity to pharmacological blocks, single-channel conductance kinetics, and voltage-dependence.On the basis of their voltage activation properties, voltage-gated Ca2+ subtypes can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels2. The activity of the channel pore is modulated by 4 tightly-coupled subunits: an intracellular β subunit; a transmembrane γ subunit; and a disulphide-linked complex of α2 and δ subunits3. There are four distinct β subunits: β1, β2, β3 and β44. There are 4 splice variants of the β2 subunit: β2a, β2b, β2c and β2d. β2a and β2b are expressed in heart, aorta and brain, and are 606- and 632-residue proteins, respectively. β2c and β2d (655 and 604 residues, respectively) are expressed in brain, and recent studies show beta-2c to be also expressed in heart. All splice variants differ in their N-terminal regions5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

CaVβ2, Voltage-dependent L-type calcium channel subunit beta-2, CACNLB2, CAB2, CACB2, Lambert-Eaton myasthenic syndrome antigen B, MYSB

Short Description :

A Rabbit Polyclonal Antibody to CACNB2 (CaVβ2) Subunit

Long Description :

Anti-CACNB2 Antibody (#ACC-105) is a highly specific antibody directed against an epitope of rat CaVβ2. The antibody can be used in western blot analysis. It has been designed to recognize CaVβ2 from rat, mouse, and human samples.

Negative Control :

BLP-CC105

Positive Control :

Synonyms :

CaVβ2, Voltage-dependent L-type calcium channel subunit beta-2, CACNLB2, CAB2, CACB2, Lambert-Eaton myasthenic syndrome antigen B, MYSB

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNB2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage