Anti-CACNB2-ATTO Fluor-594 Antibody | Alomone Labs ACC-105-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNB2-ATTO Fluor-594 Antibody

catalog :

ACC-105-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Morgenstern T, Park J, Fan Q, Colecraft H. A potent voltage-gated calcium channel inhibitor engineered from a nanobody targeted to auxiliary CaVβ subunits. elife. 2019;8: pubmed publisher

image

image 1 :

Expression of CaVβ2 in rat brain - Immunohistochemical staining of rat hippocampus using Anti-CACNB2-ATTO Fluor-594 Antibody (#ACC-105-AR). CaVβ2 staining (red) appears in CA3 pyramidal layer (arrows). Nuclei are stained using DAPI (blue).

image 2 :

Expression of CaV?2 in rat brain - Immunohistochemical staining of rat hippocampus using Anti-CACNB2-ATTO-594 Antibody(#ACC-105-AR). CaV?2 staining (red) appears in CA3 pyramidal layer (arrows). Nuclei are stained using DAPI (blue).

product information

CAT :

ACC-105-AR

SKU :

ACC-105-AR_50 mcl

Product Name :

Anti-CACNB2-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8VGC3

Applications :

IF IHC

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC105

Homology :

Mouse - identical; human - 11/17 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HEHVDRYVPHREHNHRE, corresponding to amino acid residues 571-587 of rat CACNB2

Product Page - Scientific background :

Voltage-dependent Ca2+ channels are a family of membrane proteins that allow cells to couple electrical activity to intracellular Ca2+ signaling1. Voltage-gated Ca2+ channels are classified as T, L, N, P, Q and R, and are distinguished by their sensitivity to pharmacological blocks, single-channel conductance kinetics, and voltage-dependence.On the basis of their voltage activation properties, voltage-gated Ca2+ subtypes can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels2. The activity of the channel pore is modulated by 4 tightly-coupled subunits: an intracellular β subunit; a transmembrane γ subunit; and a disulphide-linked complex of α2 and δ subunits3.There are four distinct β subunits: β1, β2, β3 and β44. There are 4 splice variants of the β2 subunit: β2a, β2b, β2c and β2d. β2a and β2b are expressed in heart, aorta and brain, and are 606- and 632-residue proteins, respectively. β2c and β2d (655 and 604 residues, respectively) are expressed in brain, and studies show beta-2c to be also expressed in heart. All splice variants differ in their N-terminal regions5.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

CaVβ2, Voltage-dependent L-type calcium channel subunit beta-2, CACNLB2, CAB2, CACB2, Lambert-Eaton myasthenic syndrome antigen B, MYSB

Short Description :

A Rabbit Polyclonal Antibody to CACNB2 (CaVβ2) Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-CACNB2 Antibody (#ACC-105) is a highly specific antibody directed against an epitope of rat CaVβ2. The antibody can be used in western blot analysis. It has been designed to recognize CaVβ2 from rat, mouse, and human samples. Anti-CACNB2-ATTO Fluor-594 Antibody (#ACC-105-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-CACNB2-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-CC105

Positive Control :

Synonyms :

CaVβ2, Voltage-dependent L-type calcium channel subunit beta-2, CACNLB2, CAB2, CACB2, Lambert-Eaton myasthenic syndrome antigen B, MYSB

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNB2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-105), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage