Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody | Alomone Labs ACC-104 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody

catalog :

ACC-104

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Toft Bertelsen T, Ziomkiewicz I, Houy S, Pinheiro P, Sørensen J. Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters. Mol Biol Cell. 2016;27:3329-3341 pubmed

image

image 1 :

Western blot analysis of rat DRG (lanes 1 and 3) and rat PC12 pheochromocytoma cell lysates (lanes 2 and 4): - 1,2. Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104), (1:200).3,4. Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody, preincubated with CACNA2D4/Cavα2δ4 (extracellular) Blocking Peptide (#BLP-CC104).

image 2 :

Expression of CaVα2δ4 in rat DRG - Immunohistochemical staining of rat DRG frozen section using Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104), (1:100). A. CaVα2δ4 is expressed in DRG neurons. B. Nuclear staining using Hoechst 33342 as the counterstain. C. Merged images of A and B.

image 3 :

Expression of CaV?2?4 in rat C6 cells - Cell surface detection ofCaV?2?4 in intact living rat brain glioma (C6) cells. A. Extracellular staining of cells withAnti-CACNA2D4 (CaV?2?4) (extracellular) Antibody(#ACC-104) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342 (blue). C. Merged images of A and B.

product information

CAT :

ACC-104

SKU :

ACC-104-CF_0.2 ml

Product Name :

Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q5RJF7

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC104

Homology :

Rat - 15/16 amino acid residues identicalHuman - not recommended for use with human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)SERPQEMGRLLGEADG, corresponding to amino acid residues 881-896 of mouse CaVα2δ4

Product Page - Scientific background :

Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. Based on their electrophysiological and pharmacological properties, Ca2+ channels have traditionally been classified into L, T, N, P, Q, and R types2. L-type calcium channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3. The Ca2+ channel α2δ subunit is a heavily glycosylated protein that is encoded by a single gene and post-translationally cleaved to yield α2 and δ subunits linked by a disulfide bond with a single transmembrane segment4.The α2δ subunit regulates many functional aspects of Ca2+ channels, such as gating, regulating voltage dependent kinetics, and increasing functional channel density on the plasma membrane5. There are four proteins that comprise CaVα2δ: CaVα2δ1, CaVα2δ2, CaVα2δ3 and CaVα2δ46. CaVα2δ4 participates in the regulation of membrane expression of CaV channels. It is predominantly expressed in certain types of endocrine cells. It is also detected in the erythroblasts in the fetal liver, in the cells of the zona reticularis of the adrenal gland and in the basophiles of the pituitary. Defects in CaVα2δ4 are the cause of retinal cone dystrophy 4 (RCD4). RCD4 is characterized by minimal symptoms except for slowly progressive reduction in visual acuity7.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4

Short Description :

A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit

Long Description :

Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It is especially suited to detect CaVα2δ4 in living cells. It has been designed to recognize CaVα2δ4 from rat and mouse samples.

Negative Control :

BLP-CC104

Positive Control :

Synonyms :

Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA2D4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage