Anti-CACNA2D4 (CaVα2δ4) (extracellular)-ATTO Fluor-594 Antibody | Alomone Labs ACC-104-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNA2D4 (CaVα2δ4) (extracellular)-ATTO Fluor-594 Antibody

catalog :

ACC-104-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

mouse, rat

application :

immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of CaV?2?4 in rat PC-12 cells - Cell surface detection ofCaV?2?4 in intact living PC-12 cells. A B. Extracellular staining of cells withAnti-CACNA2D4 (CaV?2?4) (extracellular)-ATTO-594 Antibody(#ACC-104-AR) (red) (1:25). DAPI is used as the counterstain (blue).

image 2 :

Expression of thromboxane A2 receptor in mouse cortex and hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Thromboxane A2 Receptor Antibody (#APR-069) (1:200) followed by goat anti-rabbit-AlexaFluor-488. A. TBXA2R immunoreactivity (green) is observed in mouse parietal cortex neurons (arrows). B. TBXA2R immunoreactivity (green) is detected in neurons (horizontal arrows) of mouse hippocampal CA3 pyramidal layer (P). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACC-104-AR

SKU :

ACC-104-AR_50 mcl

Product Name :

Anti-CACNA2D4 (CaVα2δ4) (extracellular)-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q5RJF7

Applications :

IC IF LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC104

Homology :

Rat - 15/16 amino acid residues identicalHuman - not recommended for use with human samples

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)SERPQEMGRLLGEADG, corresponding to amino acid residues 881-896 of mouse CaVα2δ4

Product Page - Scientific background :

Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. Based on their electrophysiological and pharmacological properties, Ca2+ channels have traditionally been classified into L, T, N, P, Q, and R types2. L-type Ca2+ channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3. The Ca2+ channel α2δ subunit is a heavily glycosylated protein that is encoded by a single gene and post-translationally cleaved to yield α2 and δ subunits linked by a disulfide bond with a single transmembrane segment4.The α2δ subunit regulates many functional aspects of Ca2+ channels, such as gating, regulating voltage dependent kinetics, and increasing functional channel density on the plasma membrane5. There are four proteins that comprise CaVα2δ: CaVα2δ1, CaVα2δ2, CaVα2δ3 and CaVα2δ46. CaVα2δ4 participates in the regulation of membrane expression of CaV channels. It is predominantly expressed in certain types of endocrine cells. It is also detected in the erythroblasts in the fetal liver, in the cells of the zona reticularis of the adrenal gland and in the basophiles of the pituitary7. Defects in CaVα2δ4 are the cause of retinal cone dystrophy 4 (RCD4). RCD4 is characterized by minimal symptoms except for slowly progressive reduction in visual acuity8.

Applications may also work in :

IC IF LCI

Supplier :

Alomone Labs

Target :

Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4

Short Description :

A Rabbit Polyclonal Antibody to CACNA2D4 (CaVα2δ4) Subunit Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize CaVα2δ4 from rat and mouse samples. Anti-CACNA2D4 (CaVα2δ4) (extracellular)-ATTO Fluor-594 Antibody (#ACC-104-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-CACNA2D4 (CaVα2δ4) (extracellular)-ATTO Fluor-594 Antibody has been tested in immunocytochemistry and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-CC104

Positive Control :

Synonyms :

Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA2D4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-104), and immunocytochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage