Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody | Alomone Labs ACC-102 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody

catalog :

ACC-102

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Sun W, Larson M, Kiyoshi C, Annett A, Stalker W, Peng J, et al. Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury. J Clin Invest. 2019;: pubmed publisher

image

image 1 :

Western blot analysis of rat lung (lanes 1 and 6), rat brain (lanes 2 and 7), mouse brain (lanes 3 and 8), rat heart (lanes 4 and 9) and mouse heart (lanes 5 and 10) lysates: - 1-5. Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102), (1:200).6-10. Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody, preincubated with CACNA2D2/Cavα2δ2 (extracellular) Blocking Peptide (#BLP-CC102).

image 2 :

Expression of CaVα2δ2 channel in rat cerebellum - Immunohistochemical staining rat cerebellum using Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102). A. CaVα2δ2 (green) appears in the soma of Purkinje cells (arrow) and in the molecular layer (M). B. Preincubation of the antibody with the blocking peptide blocks staining of Purkinje cells and molecular layer. DAPI is used as the counterstain (blue).

image 3 :

Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line (lanes 2 and 4)lysates: - 12. Anti-Homer3 Antibody (#APZ-028) (1:200).34. Anti-Homer3 Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-102

SKU :

ACC-102-CF_0.2 ml

Product Name :

Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8CFG6

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC102

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DLEAWAEKFKVLASNR, corresponding to amino acid residues 850-865 of rat CaVα2δ2

Product Page - Scientific background :

Voltage-gated Ca2+ channels (CaV), enable the passage of Ca2+ ions in a voltage-dependent manner. These heteromeric entities are formed in part by the pore-forming α1 subunit which determines the biophysical and pharmacological properties of the channel1.CaV1 and CaV2 channels are high-voltage activated (HVA) CaV channels. The a1 subunit of these channels normally interacts and associates with α2δ subunit, a membrane anchored protein and CaVβ, a cytosolic protein1.Four α2δ subunits have been cloned to date: α2δ1-4. This subunit originates from a single gene. The corresponding protein is modified by post-translational cleavage yielding a α2 subunit which is disulfide bonded to the δ subunit2. All α2δ subunits are GPI- (glycosylphosphatidylinositol) anchored proteins3. The role of this subunit is important for the membrane trafficking of the α1 subunit, and also has a role in influencing the biophysical properties of the channel1.α2δ can be expressed as various splice variants and expressed in a tissue specific manner. α2δ2 can be detected in the brain, heart, lung, spleen and liver4.Gabapentin and pregabalin are two commonly used anti-epileptic drugs. They act on CaV channels via the α2δ1 and α2δ2 subunits by disturbing their membrane trafficking, thereby decreasing Ca2+ currents.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Voltage-dependent calcium channel subunit alpha-2/delta-2

Short Description :

A Rabbit Polyclonal Antibody to CACNA2D2 (CaVα2δ2) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat CaVα2δ2. Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102) can be used in western blot and immunohistochemistry applications. It has been designed to recognize CaVα2δ2 from rat, mouse and human samples.

Negative Control :

BLP-CC102

Positive Control :

Synonyms :

Voltage-dependent calcium channel subunit alpha-2/delta-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA2D2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage