Anti-CALHM1 Antibody | Alomone Labs ACC-101 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CALHM1 Antibody

catalog :

ACC-101

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Galvanetto N, Ye Z, Marchesi A, Mortal S, Maity S, Laio A, et al. Unfolding and identification of membrane proteins in situ. elife. 2022;11: pubmed publisher
Kashio M, Wei Qi G, Ohsaki Y, Kido M, Taruno A. CALHM1/CALHM3 channel is intrinsically sorted to the basolateral membrane of epithelial cells including taste cells. Sci Rep. 2019;9:2681 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human U-87 MG brain glioblastoma cell line (lanes 3 and 7) and rat C6 brain glioma cell line (lanes 4 and 8) lysates: - 1-4. Anti-CALHM1 Antibody (#ACC-101), (1:200).5-8. Anti-CALHM1 Antibody, preincubated with CALHM1 Blocking Peptide (#BLP-CC101).

image 2 :

Expression of CALHM1 in rat hippocampus - Immunohistochemical staining of rat hippocampus using Anti-CALHM1 Antibody (#ACC-101). A. Staining of CALHM1 appears only in the hippocampal CA1 pyramidal layer (green). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge of A and B.

image 3 :

Peptide (C)EAMNHDLELGHTHG corresponding to amino acid residues 252-265 of human CALHM1 (AccessionQ8IU99). Intracellular C-terminus.

product information

CAT :

ACC-101

SKU :

ACC-101-CF_0.2 ml

Product Name :

Anti-CALHM1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8IU99

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC101

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human CALHM1

Product Page - Scientific background :

A misbalance in Ca2+ homeostasis seems to be related to the development of Alzheimer's disease (AD). Indeed, neuronal Ca2+ balance may affect the levels of proteins associated with AD, such as amyloid-beta (Ab) and tau1.Calcium homeostasis modulator 1 (CALHM1) is a cerebral Ca2+ channel component responsible for controlling intracellular Ca2+ levels and Ab metabolism2,3. CALHM1 is a three transmembrane glycoprotein, mostly localized to the endoplasmic reticulum although it can be detected at the plasma membrane. A functional channel is formed by multiple subunits which has some structural similarities to the NMDA receptor. It is expressed in all brain regions1.In cultured cells, overexpression of CALHM1 increases intracellular Ca2+ levels and reduces Ab accumulation, thereby reinforcing its implication in the development of AD. Indeed, a polymorphism in the gene was found to affect the onset of AD2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Calcium homeostasis modulator 1, FAM26C

Short Description :

A Rabbit Polyclonal Antibody to Calcium Homeostasis Modulator 1

Long Description :

Anti-CALHM1 Antibody (#ACC-101) is a highly specific antibody directed against an epitope of the human calcium homeostasis modulator protein 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CALHM1 from rat, mouse and human samples.

Negative Control :

BLP-CC101

Positive Control :

Synonyms :

Calcium homeostasis modulator 1, FAM26C

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CALHM1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage