Anti-CALHM1-ATTO Fluor-594 Antibody | Alomone Labs ACC-101-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CALHM1-ATTO Fluor-594 Antibody

catalog :

ACC-101-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of CALHM1 in rat hippocampus - Immunohistochemical staining of rat hippocampus using Anti-CALHM1-ATTO Fluor-594 Antibody (#ACC-101-AR). A. Staining of CALHM1 appears only in the hippocampal CA1 pyramidal layer. B. Nuclear staining using DAPI as the counterstain. C. Merge of A and B.

image 2 :

Expression of CALHM1 in rat hippocampus - Immunohistochemical staining of rat hippocampus using Anti-CALHM1-ATTO-594 Antibody(#ACC-101-AR). A. Staining ofCALHM1 appears only in the hippocampal CA1 pyramidal layer. B. Nuclear staining using DAPI as the counterstain. C. Merge of A and B.

product information

CAT :

ACC-101-AR

SKU :

ACC-101-AR_50 mcl

Product Name :

Anti-CALHM1-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8IU99

Applications :

IC IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC101

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

0.8 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human CALHM1

Product Page - Scientific background :

A misbalance in Ca2+ homeostasis seems to be related to the development of Alzheimer's disease (AD). Indeed Neuronal Ca2+ balance may affect the levels of proteins associated with AD, such as amyloid-beta (Ab) and tau1.Calcium homeostasis modulator 1 (CALHM1) is a cerebral Ca2+ channel component responsible for controlling intracellular Ca2+ levels and Ab metabolism2,3. CALHM1 is a three transmembrane glycoprotein, mostly localized to the endoplasmic reticulum (ER) although it can be detected at the plasma membrane. A functional channel is formed by multiple subunits which has some structural similarities to the NMDA receptor. It is expressed in all brain regions1.In cultured cells, overexpression of CALHM1 increases intracellular Ca2+ levels and reduces Ab accumulation, thereby reinforcing its implication in the development of AD. Indeed, a polymorphism in the gene was found to affect the onset of AD2.

Applications may also work in :

IC IF IHC

Supplier :

Alomone Labs

Target :

Calcium homeostasis modulator 1, FAM26C

Short Description :

A Rabbit Polyclonal Antibody to Calcium Homeostasis Modulator 1 Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-CALHM1 Antibody (#ACC-101) is a highly specific antibody directed against an epitope of the human calcium homeostasis modulator protein 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CALHM1 from rat, mouse, and human samples. Anti-CALHM1-ATTO Fluor-594 Antibody (#ACC-101-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-CALHM1-ATTO Fluor-594 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-CC101

Positive Control :

Synonyms :

Calcium homeostasis modulator 1, FAM26C

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CALHM1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-101), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage