Anti-TRPML1 (Mucolipin 1) Antibody | Alomone Labs ACC-081 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPML1 (Mucolipin 1) Antibody

catalog :

ACC-081

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Scorza S, Milano S, Saponara I, Certini M, De Zio R, Mola M, et al. TRPML1-Induced Lysosomal Ca2+ Signals Activate AQP2 Translocation and Water Flux in Renal Collecting Duct Cells. Int J Mol Sci. 2023;24: pubmed publisher
Sun J, Liu Y, Hao X, Lin W, Su W, Chiang E, et al. LAMTOR1 inhibition of TRPML1-dependent lysosomal calcium release regulates dendritic lysosome trafficking and hippocampal neuronal function. EMBO J. 2022;41:e108119 pubmed publisher
Henao J, Grismaldo A, Barreto A, Rodríguez Pardo V, Mejía Cruz C, Leal Garcia E, et al. TRPM8 Channel Promotes the Osteogenic Differentiation in Human Bone Marrow Mesenchymal Stem Cells. Front Cell Dev Biol. 2021;9:592946 pubmed publisher
Thakore P, Pritchard H, Griffin C, Yamasaki E, Drumm B, Lane C, et al. TRPML1 channels initiate Ca2+ sparks in vascular smooth muscle cells. Sci Signal. 2020;13: pubmed publisher
Naso F, Intartaglia D, Falanga D, Soldati C, Polishchuk E, Giamundo G, et al. Light-responsive microRNA miR-211 targets Ezrin to modulate lysosomal biogenesis and retinal cell clearance. EMBO J. 2020;39:e102468 pubmed publisher
Yu L, Zhang X, Yang Y, Li D, Tang K, Zhao Z, et al. Small-molecule activation of lysosomal TRP channels ameliorates Duchenne muscular dystrophy in mouse models. Sci Adv. 2020;6:eaaz2736 pubmed publisher
Park H, Gholam Zadeh M, Suh J, Choi H. Lycorine Attenuates Autophagy in Osteoclasts via an Axis of mROS/TRPML1/TFEB to Reduce LPS-Induced Bone Loss. Oxid Med Cell Longev. 2019;2019:8982147 pubmed publisher
Tedeschi V, Petrozziello T, Sisalli M, Boscia F, Canzoniero L, Secondo A. The activation of Mucolipin TRP channel 1 (TRPML1) protects motor neurons from L-BMAA neurotoxicity by promoting autophagic clearance. Sci Rep. 2019;9:10743 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 2) and HEK-293 cells (lanes 3 and 4) lysates: - 1,3. Anti-TRPML1 (Mucolipin 1) Antibody (#ACC-081), (1:200).2,4. Anti-TRPML1 (Mucolipin 1) Antibody, preincubated with TRPML1/Mucolipin 1 Blocking Peptide (#BLP-CC081).

image 2 :

Peptide (C)GRRASETERLLTPN corresponding to amino acid residues 6-19 of mouse TRPLM1 (AccessionQ99J21). Intracellular N-terminus (cytoplasmic).

image 3 :

Western blot analysis of mouse brain lysate: - 1.Anti-MAGI2/AIP1 Antibody (#APZ-052) (1:400). 2. Anti-MAGI2/AIP1 Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-081

SKU :

ACC-081-CF_0.2 ml

Product Name :

Anti-TRPML1 (Mucolipin 1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q99J21

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC081

Homology :

Human, rat - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus (cytoplasmic)

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GRRASETERLLTPN, corresponding to amino acid residues 6-19 of mouse TRPLM1

Product Page - Scientific background :

The endolysosome system takes part in important cellular functions such as membrane trafficking, protein transport, autophagy and signal transduction1. Endosomes result from endocytosis of the plasma membrane and lysosomes (which are derived from late endosomes) conatin mainly hydrolytic enzymes and generally have a low internal pH1. Like the endoplasmic reticulum (ER), endolysosomes also store Ca2+ (luminal Ca2+ concentration: 0.5 mM)1,2, and similarly to Ca2+ release from the ER, Ca2+ from endolysosomes may also play an important role in various signaling events. To date such candidates include members of the TRP super-family of ion channels and the two-pore Ca2+ channels (TPCs)1,3,4.TRPMLs, also termed mucolipins, are members of the TRP channels. In mammals, three TRPMLs are known to date (TRPML1-3 or MCOLN1-3). They are all localized to endolysosomes, although when over expressed in heterologous systems, TRPML3 is found on the plasma membrane1,5. These channels are Ca2+ permeable and display inward rectifying current properties1,5. Like all members of this family, TRPMLs have six transmembrane domains and intracellular N- and C-termini (relatively short tails compared to other members). They are characterized by an exceptionally large extracellular (luminal) loop between transmembrane domains 1 and 2, and N-glycosylation sites are present in the first extracellular (luminal) loop5.In mammals, TRPML1 is expressed in a ubiquitous manner and shows highest expression in the brain, kidney, spleen, liver and heart1,6. TRPML2 and TRPML3 are less widely expressed. Interestingly, in mouse, two splice variants exist for TRPML2. The shorter variant is more broadly expressed and is dominant over the longer variant in the thymus, spleen and kidney1,7. TRPML3 is highly detected in the thymus, lung, kidney, spleen and eye1,7,8, some epithelial cells1,9 and brain10.Pathologies related to these channels include type IV mucolipidosis, a neurodegenetative disease characterized by retardation and retinal degeneration caused by a loss of function mutation in the gene encoding TRPML1. In contrast, a gain of function mutation in TRPML3, in mice, causes deafness, and pigmentation defects11.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Mcoln1, Mucolipidin

Short Description :

A Rabbit Polyclonal Antibody to TRPML1 (Mucolipin 1) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse TRPML1. Anti-TRPML1 (Mucolipin 1) Antibody (#ACC-081) can be used in western blot analysis. It has been designed to recognize TRPML1 from human, rat and mouse samples.

Negative Control :

BLP-CC081

Positive Control :

Synonyms :

Mcoln1, Mucolipidin

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MCOLN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage