Anti-TPCN2 Antibody | Alomone Labs ACC-072 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TPCN2 Antibody

catalog :

ACC-072

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Li K, Xu J, Xue K, Yu R, Li C, Fei W, et al. Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle. Chin Med J (Engl). 2022;135:447-455 pubmed publisher
Trufanov S, Rybakova E, Avdonin P, Tsitrina A, Zharkikh I, Goncharov N, et al. The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction. Cells. 2019;8: pubmed publisher
Zapatero Belinchón F, Dietzel E, Dolnik O, Döhner K, Costa R, Hertel B, et al. Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors. Viruses. 2019;11: pubmed publisher
Rahman F, Park D, Joe Y, Jang K, Chung H, Kim U. Critical roles of Carbon Monoxide and Nitric Oxide in Ca2+ Signaling for Insulin Secretion in Pancreatic Islets. Antioxid Redox Signal. 2018;: pubmed publisher
Notomi T, Kuno M, Hiyama A, Nozaki T, Ohura K, Ezura Y, et al. Role of lysosomal channel protein TPC2 in osteoclast differentiation and bone remodeling under normal and low-magnesium conditions. J Biol Chem. 2017;292:20998-21010 pubmed publisher

image

image 1 :

Western blot analysis of mouse kidney lysate (lanes 1 and 4), rat lung membrane (lanes 2 and 5) and human embryonic Kidney 293 cell lysate (lanes 3 and 6): - 1-3. Anti-TPCN2 Antibody (#ACC-072), (1:200).4-6. Anti-TPCN2 Antibody, preincubated with TPCN2 Blocking Peptide (#BLP-CC072).

image 2 :

Expression of Two pore calcium channel protein 2 in rat cerebellum - Immunohistochemical staining of Two pore calcium channel protein 2 in rat cerebellum using Anti-TPCN2 Antibody (#ACC-072). A. TPCN2 positive cells (green) appear in the granule cell layer (G) of the cerebellum (arrows). B. The extent of the granule layer is demonstrated with the counterstain DAPI (blue).

image 3 :

Expression of Two pore calcium channel protein 2 in rat PC12 cells - Cell surface detection ofTwo pore calcium channel protein 2 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells usingAnti-TPCN2 Antibody (#ACC-072) (1:50) (red). B. Merge of A with the live view of the cell.

product information

CAT :

ACC-072

SKU :

ACC-072-CF_0.2 ml

Product Name :

Anti-TPCN2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BWC0

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC072

Homology :

Mouse, rat, human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd luminal loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

KKTLKSIRW(S)LPE(C), corresponding to amino acid residues 187-199 of mouse TPCN2 with replacement of amino acid 192 with serine (S)

Product Page - Scientific background :

Among various vertebrate species, three genes are known to encode two-pore segment channels (TPCs) termed TPC1-3. Interestingly TPC3 seems to be absent from the genomes of primates and rodents1. The primary sequence of these channels indicates the presence of two putative pore-forming repeats. Each repeat contains six transmembrane domains and a pore loop, a structure strikingly reminiscent of many voltage-gated Na+ (NaV) and Ca2+ (CaV) channels2. These twelve transmembrane structures are further thought to form functional dimers3. Both TPC1 and TPC2 show ubiquitous expression, while that of TPC1 is exceptionally high in spleen, lung, liver, and kidney2.Ca2+-mobilizing messengers such as inositol triphosphate, cyclic ADP ribose and nicotinic acid adenosine dinucleotide phosphate (NAADP) are responsible for the intracellular changes in Ca2+ ion concentration4.In contrast to the other Ca2+-mobilizing agents, NAADP, the most potent of these Ca2+ releasing molecules increases the cytosolic Ca2+ concentration via Ca2+ channels located on acidic vesicles (endolysosomes)5,6. Only quite recently, after almost a decade of being cloned, TPC1 and TPC2 were both found to be responsible for the NAADP-induced release of Ca2+ 7,8. Evidence that these two channels are indeed responsible for the release of Ca2+ is quite compelling since overexpression of TPC1 and its knockdown or point mutation of a critical residue increase and exacerbate Ca2+ release respectively7. In addition, b-cells from TPC2 knockout mice exhibited no Ca2+ release from endolysosomes upon NAADP stimulation8. Finally, in a study using immunopurified channels, it was demonstrated that TPC1 and TPC2 both respond to very low concentrations of NAADP and are unequivocally responsible for the release of Ca2+, whereas TPC3 may negatively regulate the release of Ca2+ 9.As these channels have only recently been discovered, very little is known about their physiology and gating mechanisms. Their probable involvement in a number of diseases such as lysosomal storage disease (LSDs), caused by the dysfunction of lysosomal associated proteins, has yet to be deciphered1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Two pore calcium channel protein 2, Voltage-dependent calcium channel protein TPC2, SHEP10

Short Description :

A Rabbit Polyclonal Antibody to Two Pore Calcium Channel Protein 2

Long Description :

Anti-TPCN2 Antibody (#ACC-072) is a highly specific antibody directed against an epitope of the mouse Two pore calcium channel protein 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TPCN2 from mouse, rat, and human samples.

Negative Control :

BLP-CC072

Positive Control :

Synonyms :

Two pore calcium channel protein 2, Voltage-dependent calcium channel protein TPC2, SHEP10

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TPCN2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage