Anti-TMEM66 Antibody | Alomone Labs ACC-067 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TMEM66 Antibody

catalog :

ACC-067

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat pancreas (lanes 1 and 2), Mouse MS1 endothelial cells (lanes 3 and 5) and human PANC1 pancreatic carcinoma cells (lanes 4 and 6) lysates: - 1,3,4. Anti-TMEM66 Antibody (#ACC-067), (1:500).2,5,6. Anti-TMEM66 Antibody, preincubated with TMEM66 Blocking Peptide (#BLP-CC067).

image 2 :

Expression of TMEM66 in mouse cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TMEM66 Antibody (#ACC-067), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. TMEM66 immunoreactivity (green) appears in the molecular layer (MOL) and in neurons of the purkinje cell layer (P, arrows). B. Pre-incubation of the antibody with TMEM66 Blocking Peptide (#BLP-CC067), suppresses staining. Cell nuclei are stained with DAPI (blue). G = granule layer.

image 3 :

Cell surface detection of CCR9 in live intact rat lymphoma T-lymphocyte (C58) cells: - ___Cells.___Cells + goat-anti-rabbit-FITC.___Cells +Anti-CCR9 (extracellular) Antibody (#ACR-029) (5 ?g/0.5x106 cells) + goat anti-rabbit-FITC.

product information

CAT :

ACC-067

SKU :

ACC-067-CF_0.2 ml

Product Name :

Anti-TMEM66 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6AYN2

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC067

Homology :

Mouse - 13/15 amino acid residues identical; human - 11/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, lumen.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ELGLSKLKESGKHQS, corresponding to amino acid residues 141-155 of rat SARAF

Product Page - Scientific background :

TMEM66 (Transmembrane protein 66, also known as SOCE-associated regulatory factor, SARAF) is a regulatory protein of cellular Ca2+ homeostasis, encoded by the TMEM66 gene. TMEM66 is an ER resident protein, which responds to cytosolic Ca2+ elevation after ER Ca2+ refilling.TMEM66 is highly conserved in vertebrates. In mammals it is ubiquitously expressed with high transcript levels in the immune and neuronal tissues. The TMEM66 structure contains validated signal peptide and a putative single membrane spanning domain, a serine-proline rich domain and arginine rich regions followed by a cluster of basic residues at its C-terminal tail1.Store-operated Ca2+ entry (SOCE) is an important Ca2+ influx pathway across plasma membranes in many non-excitable cells. In this pathway, intracellular Ca2+ release through IP3 receptors stimulated by a variety of agonists, results in reduction of Ca2+ concentration in the lumen of the endoplasmic or sarcoplasmic reticulum. SARAF negatively regulates store operated calcium entry into cells and protects cells from calcium overfilling1,2.STIM, Ca2+ sensor, and Orai, the channel pore forming subunit proteins have been identified as the essential components enabling the reconstitution of Ca2+ release-activated Ca2+ channels that mediate SOCE. Knock-out of Orai1 or STIM1 in mice and mutations evoke SOCE in lymphocytes and other cells2,3.Findings show that dysregulation of Ca2+ homeostasis is associated with a plethora of pathological conditions including neurodegenerative diseases, skeletal, muscular and cardiovascular disorders2. Inherited defects in SOCE due to mutations in genes of the Ca2+ release-activated Ca2+ (CRAC) channel complex causes patients to suffer from a severe form of immunodeficiency that is due to defects in the function of T cells, NK cells and potentially other immune cells3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66

Short Description :

A Rabbit Polyclonal Antibody to TMEM66

Long Description :

Anti-TMEM66 Antibody (#ACC-067) is a highly specific antibody directed against an epitope of rat transmembrane protein 66. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TMEM66 from human, rat, and mouse samples.

Negative Control :

BLP-CC067

Positive Control :

Synonyms :

Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SARAF

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage