Anti-Orai3 Antibody | Alomone Labs ACC-065 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Orai3 Antibody

catalog :

ACC-065

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 1h	Kim J, Hwang K, Dang B, Eom M, Kong I, Gwack Y, et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 2021;12:6537 pubmed publisher
	Chaki S, Alkanfari I, Roy S, Amponnawarat A, Hui Y, Oskeritzian C, et al. Inhibition of Orai Channel Function Regulates Mas-Related G Protein-Coupled Receptor-Mediated Responses in Mast Cells. Front Immunol. 2021;12:803335 pubmed publisher
	Bittremieux M, Gerasimenko J, Schuermans M, Luyten T, Stapleton E, Alzayady K, et al. DPB162-AE, an inhibitor of store-operated Ca2+ entry, can deplete the endoplasmic reticulum Ca2+ store. Cell Calcium. 2017;62:60-70 pubmed publisher
	Sabourin J, Bartoli F, Antigny F, Gomez A, Benitah J. Transient Receptor Potential Canonical (TRPC)/Orai1-dependent Store-operated Ca2+ Channels: NEW TARGETS OF ALDOSTERONE IN CARDIOMYOCYTES. J Biol Chem. 2016;291:13394-409 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart membranes: - 1,2. Anti-Orai3 Antibody (#ACC-065), (1:800).3,4. Anti-Orai3 Antibody, preincubated with Orai3 Blocking Peptide (#BLP-CC065).

image 2 :

Peptide (C)REFVHRGYLDLMGAS corresponding to amino acid residues 28-42 of rat Orai3 (AccessionQ6AXR8). Intracellular N-terminus.

image 3 :

Peptide KKGWMDPQSKGIQTGRC corresponding to amino acid residues 136-152 of mouse P2X7 receptor (AccessionQ9Z1M0). Extracellular loop.

product information

CAT :

ACC-065

SKU :

ACC-065-CF_0.2 ml

Product Name :

Anti-Orai3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6AXR8

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC065

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)REFVHRGYLDLMGAS, corresponding to amino acid residues 28-42 of rat Orai3

Product Page - Scientific background :

In non-excitable cells, Ca2+ signaling plays a most important role in important cellular functions such as migration, proliferation and differentiation1. In such cells, Ca2+ enters via either non-selective cation channels such as TRPCs or through highly selective Ca2+ such as Ca2+ release-activated Ca2+ channels (CRAC channels) or store-operated Ca2+ entry channels (SOC channels), and the arachidonic acid-regulated Ca2+ channels (ARC channels)2.Orai channels are part of the molecular components involved in the Ca2+ entry described above. Three Orai channels have been described in mammalian cells: Orai1-3. These channels make up the pore forming unit of CRAC channels3. They are membrane proteins with four transmembrane domains and intracellular N- and C-termini. Orai1 and Orai3 share similar distribution and are expressed in the heart, brain, liver, spleen, lung, intestine, lymphoid organs, skin, and skeletal muscle. Expression of Orai2 is more limited and is found mainly in the brain and lower expression is detected in the lung, spleen and intestine3.CRAC channels have been for the most part identified as homotetramers of Orai1 interacting with endoplasmic reticulum located STIM1 that are activated by a depletion of intracellular Ca2+ 4. Orai3 was shown to emit store-operated Ca2+ entry (SOCE) currents along with STIM1/2 in breast cancer cells which are Estrogen Receptor positive (ER+), whereas Orai1/STIM1 are responsible for these currents in Estrogen Receptor negative (ER-) cells1. Furthermore, inhibition of Orai3 in these cells elicits cell cycle arrest and ultimately apoptosis (normal cells do not undergo apoptosis)5. ARC channels are composed of a heteropentameric organization of Orai1/Orai3 (in a 3:2 ratio) which interacts with the small fraction of plasma membrane localized STIM1. These channels are activated by low concentrations of arachidonic acid localized at the inner face of the plasma membrane6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

TMEM142C

Short Description :

A Rabbit Polyclonal Antibody to Orai3 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Orai3. Anti-Orai3 Antibody (#ACC-065) can be used in western blot analysis. It has been designed to recognize Orai3 from human, rat, and mouse samples.

Negative Control :

BLP-CC065

Positive Control :

Synonyms :

TMEM142C

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ORAI3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage