Anti-STIM2 Antibody | Alomone Labs ACC-064 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-STIM2 Antibody

catalog :

ACC-064

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:100; loading ...; fig 1k	Kim J, Hwang K, Dang B, Eom M, Kong I, Gwack Y, et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 2021;12:6537 pubmed publisher
	Chubinskiy Nadezhdin V, Semenova S, Vasileva V, Shatrova A, Pugovkina N, Negulyaev Y. Store-Operated Ca2+ Entry Contributes to Piezo1-Induced Ca2+ Increase in Human Endometrial Stem Cells. Int J Mol Sci. 2022;23: pubmed publisher
	Zhu J, Zhang X, Xie H, Wang Y, Zhang X, Lin Z. Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress. J Inflamm Res. 2021;14:3945-3958 pubmed publisher
	Gruszczynska Biegala J, Strucinska K, Maciąg F, Majewski L, Sladowska M, Kuznicki J. STIM Protein-NMDA2 Receptor Interaction Decreases NMDA-Dependent Calcium Levels in Cortical Neurons. Cells. 2020;9: pubmed publisher
	Tiscione S, Vivas O, Ginsburg K, Bers D, Ory D, Santana L, et al. Disease-associated mutations in Niemann-Pick type C1 alter ER calcium signaling and neuronal plasticity. J Cell Biol. 2019;218:4141-4156 pubmed publisher
	Chauhan A, Liu X, Jing J, Lee H, Yadav R, Liu J, et al. STIM2 interacts with AMPK and regulates calcium-induced AMPK activation. FASEB J. 2018;:fj201801225R pubmed publisher
	Huang T, Lin Y, Chang H, Yeh T, Chang Y, Chen Y, et al. STIM1 Knockout Enhances PDGF-Mediated Ca2+ Signaling through Upregulation of the PDGFR⁻PLCγ⁻STIM2 Cascade. Int J Mol Sci. 2018;19: pubmed publisher
	Zhang W, Takahara T, Achiha T, Shibata H, Maki M. Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements Facilitates Analysis of Biological Endpoint Effects of Cellular Ca2+ Mobilization. Int J Mol Sci. 2018;19: pubmed publisher
	Gonz lez S nchez P, del Arco A, Esteban J, Satr stegui J. Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons. Front Cell Neurosci. 2017;11:363 pubmed publisher
	Sabourin J, Bartoli F, Antigny F, Gomez A, Benitah J. Transient Receptor Potential Canonical (TRPC)/Orai1-dependent Store-operated Ca2+ Channels: NEW TARGETS OF ALDOSTERONE IN CARDIOMYOCYTES. J Biol Chem. 2016;291:13394-409 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1, 3) and rat RBL basophilic leukemia cell (lanes 2, 4) lysates: - 1. Anti-STIM2 Antibody (#ACC-064), (1:200).2. Anti-STIM2 Antibody, preincubated with STIM2 Blocking Peptide (#BLP-CC064).

image 2 :

Expression of STIM2 in rat lung - Immunohistochemical staining of paraffin embedded rat lung sections using Anti-STIM2 Antibody (#ACC-064), (1:100). Staining is present in the respiratory epithelium of the bronchiole (red) as well as in the pneumonocytes of the alveolar wall (blue). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the counterstain.

image 3 :

Peptide SAEKQWEVPDTASEC corresponding to amino acid residues 583-597 of human STIM2 (AccessionQ9P246). Intracellular C-terminus.

product information

CAT :

ACC-064

SKU :

ACC-064-CF_0.2 ml

Product Name :

Anti-STIM2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9P246

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC064

Homology :

Rat, mouse, bovine - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2

Product Page - Scientific background :

Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become the focus of intense research.Recently, several key players of the store-operated complex have been identified: the Orai family consists of three members: Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4While STIM1 appears to be localized intracellularly at the ER membrane and at a much lower extent on the cell surface of several cell types, STIM2 appears to be localized solely at the ER membrane.5 The function of STIM2 still remains elusive and several models have been suggested.6-8

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Stromal interaction molecule 2

Short Description :

A Rabbit Polyclonal Antibody to STIM2

Long Description :

Anti-STIM2 Antibody (#ACC-064) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize STIM2 from human, rat, and mouse samples.

Negative Control :

BLP-CC064

Positive Control :

Synonyms :

Stromal interaction molecule 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

STIM2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage