Anti-STIM1 (extracellular) Antibody | Alomone Labs ACC-063 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-STIM1 (extracellular) Antibody

catalog :

ACC-063

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 12

Reference
Chubinskiy Nadezhdin V, Semenova S, Vasileva V, Shatrova A, Pugovkina N, Negulyaev Y. Store-Operated Ca2+ Entry Contributes to Piezo1-Induced Ca2+ Increase in Human Endometrial Stem Cells. Int J Mol Sci. 2022;23: pubmed publisher
Tiffner A, Hopl V, Schober R, Sallinger M, Grabmayr H, Höglinger C, et al. Orai1 Boosts SK3 Channel Activation. Cancers (Basel). 2021;13: pubmed publisher
Zhu J, Zhang X, Xie H, Wang Y, Zhang X, Lin Z. Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress. J Inflamm Res. 2021;14:3945-3958 pubmed publisher
Wang L, Lei Q, Zhao S, Xu W, Dong W, Ran J, et al. Ginkgolide B Maintains Calcium Homeostasis in Hypoxic Hippocampal Neurons by Inhibiting Calcium Influx and Intracellular Calcium Release. Front Cell Neurosci. 2020;14:627846 pubmed publisher
Yamamura H, Suzuki Y, Asai K, Imaizumi Y, Yamamura H. Oxidative stress facilitates cell death by inhibiting Orai1-mediated Ca2+ entry in brain capillary endothelial cells. Biochem Biophys Res Commun. 2020;523:153-158 pubmed publisher
Tedeschi V, Petrozziello T, Sisalli M, Boscia F, Canzoniero L, Secondo A. The activation of Mucolipin TRP channel 1 (TRPML1) protects motor neurons from L-BMAA neurotoxicity by promoting autophagic clearance. Sci Rep. 2019;9:10743 pubmed publisher
D Costa S, Ayyadurai S, Gibson A, Mackey E, Rajput M, Sommerville L, et al. Mast cell corticotropin-releasing factor subtype 2 suppresses mast cell degranulation and limits the severity of anaphylaxis and stress-induced intestinal permeability. J Allergy Clin Immunol. 2019;143:1865-1877.e4 pubmed publisher
Latour S, Mahouche I, Cherrier F, Azzi Martin L, Velasco V, Soubeyran P, et al. Calcium Independent Effect of Orai1 and STIM1 in Non-Hodgkin B Cell Lymphoma Dissemination. Cancers (Basel). 2018;10: pubmed publisher
Chauhan A, Liu X, Jing J, Lee H, Yadav R, Liu J, et al. STIM2 interacts with AMPK and regulates calcium-induced AMPK activation. FASEB J. 2018;:fj201801225R pubmed publisher
Zeng B, Chen G, Garcia Vaz E, Bhandari S, Daskoulidou N, Berglund L, et al. ORAI channels are critical for receptor-mediated endocytosis of albumin. Nat Commun. 2017;8:1920 pubmed publisher
García Castañeda M, Vega A, Rodríguez R, Montiel Jaen M, Cisneros B, Zarain Herzberg A, et al. Functional impact of an oculopharyngeal muscular dystrophy mutation in PABPN1. J Physiol. 2017;595:4167-4187 pubmed publisher
Zhang W, Qi Z, Wang Y. BTP2, a Store-Operated Calcium Channel Inhibitor, Attenuates Lung Ischemia-Reperfusion Injury in Rats. Inflammation. 2017;40:778-787 pubmed publisher

image

image 1 :

Western blot analysis of rat RBL basophilic leukemia (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5), and human Jurkat T-cell leukemia (lanes 3 and 6) cell lysates: - 1,2,3. Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:1000).4,5,6. Anti-STIM1 (extracellular) Antibody, preincubated with STIM1 (extracellular) Blocking Peptide (#BLP-CC063).

image 2 :

Expression of STIM1 in rat stomach - Immunohistochemical staining of paraffin embedded rat stomach sections using Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:100). STIM1 is expressed in the parietal cells of the gastric mucosa (arrows). Hematoxilin is used as the counterstain.

image 3 :

Expression of STIM1 in rat stomach - Immunohistochemical staining of paraffin embedded rat stomach sections usingAnti-STIM1(extracellular)Antibody (#ACC-063) (1:100). STIM1 is expressed in the parietal cells of the gastric mucosa (arrows). Hematoxilin is used as the counterstain.

product information

CAT :

ACC-063

SKU :

ACC-063-CF_0.2 ml

Product Name :

Anti-STIM1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q13586

Applications :

IC IF IFC IHC IP LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC063

Homology :

Rat, mouse, bovine, canis - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

STIM1 expressed on the plasma membrane: Extracellular, N-terminus STIM1 expressed on the ER: Luminal, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1

Product Page - Scientific background :

Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest.Recently, several key players of the store-operated complex have been identified.3 The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+-binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 molecules are redistributed in punctae underneath the plasma membrane and activate SOCs.Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.5 Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed.

Applications may also work in :

IC IF IFC IHC IP LCI WB

Supplier :

Alomone Labs

Target :

Stromal interaction molecule 1, GOK

Short Description :

A Rabbit Polyclonal Antibody to STIM1

Long Description :

Anti-STIM1 (extracellular) Antibody (#ACC-063) is a highly specific antibody directed against an epitope of the human Stromal interaction molecule 1. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize STIM1 from human, rat, and mouse samples.

Negative Control :

BLP-CC063

Positive Control :

Synonyms :

Stromal interaction molecule 1, GOK

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

STIM1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage