Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody | Alomone Labs ACC-063-AO product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody

catalog :

ACC-063-AO

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Kikuta S, Iguchi Y, Kakizaki T, Kobayashi K, Yanagawa Y, Takada M, et al. Store-Operated Calcium Channels Are Involved in Spontaneous Slow Calcium Oscillations in Striatal Neurons. Front Cell Neurosci. 2019;13:547 pubmed publisher
Tedeschi V, Petrozziello T, Sisalli M, Boscia F, Canzoniero L, Secondo A. The activation of Mucolipin TRP channel 1 (TRPML1) protects motor neurons from L-BMAA neurotoxicity by promoting autophagic clearance. Sci Rep. 2019;9:10743 pubmed publisher

image

image 1 :

Expression of STIM1 in rat pancreas - Immunohistochemical staining of rat paraffin-embedded pancreas sections using Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO), (1:20), (red). A. STIM1 staining is highly specific for endocrine cells in the Isle of Langerhans. B. Hoechst 33342 (blue) is used as the counterstain. C. Merged image of panels A and B.

image 2 :

Expressionof STIM1 in rat pancreas - Immunohistochemical staining of rat paraffin-embedded pancreas sections using Anti-STIM1 (extracellular)-ATTO-550Antibody (#ACC-063-AO) (1:20) (red). A. STIM1 staining is highly specific for endocrine cells in the Isle of Langerhans. B. Hoechst 33342 (blue) is used as the counterstain. C. Merged image of panels A and B.

image 3 :

Alomone Labs SKA-121 activates KCa3.1 (SK4) currents in stably transfected 293T cells. - A. Representative time course of KCa3.1 current at 0 mV recorded using whole-cell patch-clamp. Membrane potential was held at -80 mV a 150 ms voltage ramp from -120 mV to +60 mV was applied every 10 sec. Current was activated at low nanomolar [Ca2+]in by application of 1 M and 10 M SKA-121 (#S-245) as indicated. B. Superimposed traces of KCa3.1 current following application of 1 M and 10 M SKA-121 taken from the recording in A.

product information

CAT :

ACC-063-AO

SKU :

ACC-063-AO_50 mcl

Product Name :

Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q13586

Applications :

FC IC IF IHC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC063

Homology :

Rat, mouse, bovine and canis - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

STIM1 expressed on the plasma membrane: Extracellular, N-terminus STIM1 expressed on the ER: Luminal, N-terminus

Label :

ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1

Product Page - Scientific background :

Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest.Recently, several key players of the store-operated complex have been identified.3 The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+ binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 molecules are redistributed in punctae underneath the plasma membrane and activate SOCs.Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.5 Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed.

Applications may also work in :

FC IC IF IHC LCI

Supplier :

Alomone Labs

Target :

Stromal interaction molecule 1, GOK

Short Description :

A Rabbit Polyclonal Antibody to STIM1 Conjugated to the Fluorescent Dye ATTO-550

Long Description :

Anti-STIM1 (extracellular) Antibody (#ACC-063) is a highly specific antibody directed against an extracellular epitope of the human Stromal interaction molecule 1. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize STIM1 from human, rat, and mouse samples. Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AO

Positive Control :

Synonyms :

Stromal interaction molecule 1, GOK

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

STIM1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-063), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:20

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage