Anti-Orai1 (extracellular) Antibody | Alomone Labs ACC-062 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Orai1 (extracellular) Antibody

catalog :

ACC-062

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; fig 8a	García Castañeda M, Vega A, Rodríguez R, Montiel Jaen M, Cisneros B, Zarain Herzberg A, et al. Functional impact of an oculopharyngeal muscular dystrophy mutation in PABPN1. J Physiol. 2017;595:4167-4187 pubmed publisher
western blot; mouse; loading ...; fig 3c	Lisak D, Schacht T, Gawlitza A, Albrecht P, Aktas O, Koop B, et al. BAX inhibitor-1 is a Ca(2+) channel critically important for immune cell function and survival. Cell Death Differ. 2016;23:358-68 pubmed publisher
	Chaki S, Alkanfari I, Roy S, Amponnawarat A, Hui Y, Oskeritzian C, et al. Inhibition of Orai Channel Function Regulates Mas-Related G Protein-Coupled Receptor-Mediated Responses in Mast Cells. Front Immunol. 2021;12:803335 pubmed publisher
	Tiffner A, Hopl V, Schober R, Sallinger M, Grabmayr H, Höglinger C, et al. Orai1 Boosts SK3 Channel Activation. Cancers (Basel). 2021;13: pubmed publisher
	Zhu J, Zhang X, Xie H, Wang Y, Zhang X, Lin Z. Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress. J Inflamm Res. 2021;14:3945-3958 pubmed publisher
	Han J, Heo W, Lee D, Kang C, Kim H, Jun I, et al. Plasma Membrane Localized GCaMP-MS4A12 by Orai1 Co-Expression Shows Thapsigargin- and Ca2+-Dependent Fluorescence Increases. Mol Cells. 2021;44:223-232 pubmed publisher
	Pelletier L, Petiot A, Brocard J, Giannesini B, Giovannini D, Sanchez C, et al. In vivo RyR1 reduction in muscle triggers a core-like myopathy. Acta Neuropathol Commun. 2020;8:192 pubmed publisher
	Moon H, Min C, Kim G, Kim D, Kim K, Lee S, et al. Crbn modulates calcium influx by regulating Orai1 during efferocytosis. Nat Commun. 2020;11:5489 pubmed publisher
	Luo R, Gomez A, Benitah J, Sabourin J. Targeting Orai1-Mediated Store-Operated Ca2+ Entry in Heart Failure. Front Cell Dev Biol. 2020;8:586109 pubmed publisher
	Garcia S, Herbert L, Walker B, Resta T, Jernigan N. Coupling of store-operated calcium entry to vasoconstriction is acid-sensing ion channel 1a dependent in pulmonary but not mesenteric arteries. PLoS ONE. 2020;15:e0236288 pubmed publisher
	Toth A, Hori K, Novakovic M, Bernstein N, Lambot L, Prakriya M. CRAC channels regulate astrocyte Ca2+ signaling and gliotransmitter release to modulate hippocampal GABAergic transmission. Sci Signal. 2019;12: pubmed publisher
	D Costa S, Ayyadurai S, Gibson A, Mackey E, Rajput M, Sommerville L, et al. Mast cell corticotropin-releasing factor subtype 2 suppresses mast cell degranulation and limits the severity of anaphylaxis and stress-induced intestinal permeability. J Allergy Clin Immunol. 2019;143:1865-1877.e4 pubmed publisher
	Tam K, Ali E, Hua J, Chataway T, Barritt G. Evidence for the interaction of peroxiredoxin-4 with the store-operated calcium channel activator STIM1 in liver cells. Cell Calcium. 2018;74:14-28 pubmed publisher
	El Hachmane M, Ermund A, Brännmark C, Olofsson C. Extracellular ATP activates store-operated Ca2+ entry in white adipocytes: functional evidence for STIM1 and ORAI1. Biochem J. 2018;475:691-704 pubmed publisher
	Gonz lez S nchez P, del Arco A, Esteban J, Satr stegui J. Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons. Front Cell Neurosci. 2017;11:363 pubmed publisher
	Zeng B, Chen G, Garcia Vaz E, Bhandari S, Daskoulidou N, Berglund L, et al. ORAI channels are critical for receptor-mediated endocytosis of albumin. Nat Commun. 2017;8:1920 pubmed publisher
	Zhang B, Naik J, Jernigan N, Walker B, Resta T. Reduced membrane cholesterol after chronic hypoxia limits Orai1-mediated pulmonary endothelial Ca2+ entry. Am J Physiol Heart Circ Physiol. 2018;314:H359-H369 pubmed publisher
	Li H, Tan X, Yan L, Zeng B, Meng J, Xu H, et al. Multi-walled carbon nanotubes act as a chemokine and recruit macrophages by activating the PLC/IP3/CRAC channel signaling pathway. Sci Rep. 2017;7:226 pubmed publisher
	Kuang S, Qian J, Yang H, Rao F, Chen X, Zhang M, et al. The enhancement of TXA2 receptors-mediated contractile response in intrarenal artery dysfunction in type 2 diabetic mice. Eur J Pharmacol. 2017;805:93-100 pubmed publisher
	Papanikolaou M, Lewis A, Butt A. Store-operated calcium entry is essential for glial calcium signalling in CNS white matter. Brain Struct Funct. 2017;222:2993-3005 pubmed publisher
	Li J, Yan B, Si H, Peng X, Zhang S, Hu J. Atlastin regulates store-operated calcium entry for nerve growth factor-induced neurite outgrowth. Sci Rep. 2017;7:43490 pubmed publisher
	Ben Kasus Nissim T, Zhang X, Elazar A, Roy S, Stolwijk J, Zhou Y, et al. Mitochondria control store-operated Ca2+ entry through Na+ and redox signals. EMBO J. 2017;36:797-815 pubmed publisher
	Zhang W, Qi Z, Wang Y. BTP2, a Store-Operated Calcium Channel Inhibitor, Attenuates Lung Ischemia-Reperfusion Injury in Rats. Inflammation. 2017;40:778-787 pubmed publisher
	Tang B, Xia X, Lv X, Yu B, Yuan J, Mai X, et al. Inhibition of Orai1-mediated Ca2+ entry enhances chemosensitivity of HepG2 hepatocarcinoma cells to 5-fluorouracil. J Cell Mol Med. 2017;21:904-915 pubmed publisher
	Gonzalez Bosc L, Plomaritas D, Herbert L, Giermakowska W, Browning C, Jernigan N. ASIC1-mediated calcium entry stimulates NFATc3 nuclear translocation via PICK1 coupling in pulmonary arterial smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 2016;311:L48-58 pubmed publisher
	Qi Z, Wang Y, Zhou H, Liang N, Yang L, Liu L, et al. The Central Analgesic Mechanism of YM-58483 in Attenuating Neuropathic Pain in Rats. Cell Mol Neurobiol. 2016;36:1035-43 pubmed publisher

image

image 1 :

Western blot analysis of rat spleen (lanes 1 and4), rat pancreas (lanes 2 and 5) and mouse B-cell lymphoma (WEHI) (lanes 3 and 6) lysates: - 1-3. Anti-Orai1 (extracellular) Antibody (#ACC-062), (1:200).4-6. Anti-Orai1 (extracellular) Antibody, preincubated with Orai1 (extracellular) Blocking Peptide (#BLP-CC062).

image 2 :

Expression of Orai1 in rat pancreas - Immunohistochemical staining of rat pancreas paraffin embedded sections using Anti-Orai1 (extracellular) Antibody (#ACC-062), (1:50). Orai1 (brown) is expressed in acinar cells. Hematoxilin is used as the counterstain.

image 3 :

Peptide (C)KFLPLKRQAGQPS corresponding to amino acid residues 200-212 of mouse Orai1 (AccessionQ8BWG9). 2ndextracellular loop.

product information

CAT :

ACC-062

SKU :

ACC-062-CF_0.2 ml

Product Name :

Anti-Orai1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BWG9

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC062

Homology :

Rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KFLPLKRQAGQPS, corresponding to amino acid residues 200-212 of mouse Orai1

Product Page - Scientific background :

Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation1,2. Intracellular Ca2+ levels are controlled by either the influx of Ca2+ through the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores which gained much attention.Recently, several key players of the store operated complex have been identified. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Short Description :

A Rabbit Polyclonal Antibody to Orai1 Channel

Long Description :

Anti-Orai1 (extracellular) Antibody (#ACC-062) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize the Orai1 channel from mouse and rat samples.

Negative Control :

BLP-CC062

Positive Control :

Synonyms :

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ORAI1

AB Specifictiy :

Will not recognize human Orai1.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:1000

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage