Anti-Orai2 Antibody | Alomone Labs ACC-061 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Orai2 Antibody

catalog :

ACC-061

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 1h	Kim J, Hwang K, Dang B, Eom M, Kong I, Gwack Y, et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 2021;12:6537 pubmed publisher
western blot; human; 1:500; fig 1e	Zeng B, Chen G, Garcia Vaz E, Bhandari S, Daskoulidou N, Berglund L, et al. ORAI channels are critical for receptor-mediated endocytosis of albumin. Nat Commun. 2017;8:1920 pubmed publisher
	Chaki S, Alkanfari I, Roy S, Amponnawarat A, Hui Y, Oskeritzian C, et al. Inhibition of Orai Channel Function Regulates Mas-Related G Protein-Coupled Receptor-Mediated Responses in Mast Cells. Front Immunol. 2021;12:803335 pubmed publisher
	Bhandage A, Jin Z, Korol S, Tafreshiha A, Gohel P, Hellgren C, et al. Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes. PLoS ONE. 2018;13:e0208981 pubmed publisher
	He X, Song S, Ayon R, Balisterieri A, Black S, Makino A, et al. Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells. Am J Physiol Cell Physiol. 2018;314:C504-C517 pubmed publisher
	Gonz lez S nchez P, del Arco A, Esteban J, Satr stegui J. Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons. Front Cell Neurosci. 2017;11:363 pubmed publisher
	Bittremieux M, Gerasimenko J, Schuermans M, Luyten T, Stapleton E, Alzayady K, et al. DPB162-AE, an inhibitor of store-operated Ca2+ entry, can deplete the endoplasmic reticulum Ca2+ store. Cell Calcium. 2017;62:60-70 pubmed publisher

image

image 1 :

Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 3) and human Jurkat acute T cell leukemia (lanes 2 and 4) cell lysates: - 1,2. Anti-Orai2 Antibody (#ACC-061), (1:200).3,4. Anti-Orai2 Antibody, preincubated with Orai2 Blocking Peptide (#BLP-CC061).

image 2 :

Expression of Orai2 in rat lung - Immunohistochemical staining of paraffin-embedded rat lung sections using Anti-Orai2 Antibody (#ACC-061), (1:100). Strong and specific staining is evident in bronchiolar epithelium (blue) and alveoli walls (red). Hematoxilin is used as the counterstain.

image 3 :

Expression of Orai2 in rat lung - Immunohistochemical staining of paraffin-embedded rat lung sections usingAnti-Orai2Antibody (#ACC-061) (1:100). Strong and specific staining is evident in bronchiolar epithelium (blue) and alveoli walls (red). Hematoxilin is used as the counterstain.

product information

CAT :

ACC-061

SKU :

ACC-061-CF_0.2 ml

Product Name :

Anti-Orai2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BH10

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC061

Homology :

Human, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CPEPGHKGMDYRDWVRR, corresponding to amino acid residues 16-32 of mouse Orai2

Product Page - Scientific background :

Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx, either by the calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores, has become of great interest.Recently, several key players of the store-operated complex have been identified: the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum Ca2+ sensor.3,4 Orai1, Orai2, and Orai3 are all capable of forming store-operated channels.5Co-expresssion of Orai2 and STIM1 was shown to produce currents that appear similar but smaller than those produced by co-expression of Orai1 and STIM1.6Orai2 transcripts are predominantly expressed in kidney, lung, and spleen, and it appears that Orai2 is the only Orai isoform with multiple transcripts.7

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

TMEM142B

Short Description :

A Rabbit Polyclonal Antibody to Orai2 Channel

Long Description :

Anti-Orai2 Antibody (#ACC-061) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Orai2 from human, mouse, and rat samples.

Negative Control :

BLP-CC061

Positive Control :

Synonyms :

TMEM142B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ORAI2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100-1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage