Anti-Human Orai1 (extracellular) Antibody | Alomone Labs ACC-060 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human Orai1 (extracellular) Antibody

catalog :

ACC-060

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Alomone Labs product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; fig 1a western blot; human; 1:500; fig 1e	Zeng B, Chen G, Garcia Vaz E, Bhandari S, Daskoulidou N, Berglund L, et al. ORAI channels are critical for receptor-mediated endocytosis of albumin. Nat Commun. 2017;8:1920 pubmed publisher
	Chubinskiy Nadezhdin V, Semenova S, Vasileva V, Shatrova A, Pugovkina N, Negulyaev Y. Store-Operated Ca2+ Entry Contributes to Piezo1-Induced Ca2+ Increase in Human Endometrial Stem Cells. Int J Mol Sci. 2022;23: pubmed publisher
	Wu B, Ramaiah A, Garcia G, Hasiakos S, Arumugaswami V, Srikanth S. ORAI1 Limits SARS-CoV-2 Infection by Regulating Tonic Type I IFN Signaling. J Immunol. 2022;208:74-84 pubmed publisher
	Bokhobza A, Ziental Gelus N, Allart L, Iamshanova O, Vanden Abeele F. Impact of SOCE Abolition by ORAI1 Knockout on the Proliferation, Adhesion, and Migration of HEK-293 Cells. Cells. 2021;10: pubmed publisher
	Gawali V, Chimote A, Newton H, Feria Garz xf3 n M, Chirra M, Janssen E, et al. Immune Checkpoint Inhibitors Regulate K+ Channel Activity in Cytotoxic T Lymphocytes of Head and Neck Cancer Patients. Front Pharmacol. 2021;12:742862 pubmed publisher
	Wu B, Ramaiah A, Garcia G, Gwack Y, Arumugaswami V, Srikanth S. ORAI1 establishes resistance to SARS-CoV-2 infection by regulating tonic type I interferon signaling. bioRxiv. 2021;: pubmed publisher
	Movsisyan N, Pardo L. Kv10.1 Regulates Microtubule Dynamics during Mitosis. Cancers (Basel). 2020;12: pubmed publisher
	Yamamura H, Suzuki Y, Asai K, Imaizumi Y, Yamamura H. Oxidative stress facilitates cell death by inhibiting Orai1-mediated Ca2+ entry in brain capillary endothelial cells. Biochem Biophys Res Commun. 2020;523:153-158 pubmed publisher
	Tiscione S, Vivas O, Ginsburg K, Bers D, Ory D, Santana L, et al. Disease-associated mutations in Niemann-Pick type C1 alter ER calcium signaling and neuronal plasticity. J Cell Biol. 2019;218:4141-4156 pubmed publisher
	Latour S, Zanese M, Le Morvan V, Vacher A, Menard N, Bijou F, et al. Role of Calcium Signaling in GA101-Induced Cell Death in Malignant Human B Cells. Cancers (Basel). 2019;11: pubmed publisher
	Bhandage A, Jin Z, Korol S, Tafreshiha A, Gohel P, Hellgren C, et al. Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes. PLoS ONE. 2018;13:e0208981 pubmed publisher
	Latour S, Mahouche I, Cherrier F, Azzi Martin L, Velasco V, Soubeyran P, et al. Calcium Independent Effect of Orai1 and STIM1 in Non-Hodgkin B Cell Lymphoma Dissemination. Cancers (Basel). 2018;10: pubmed publisher
	Chauhan A, Sun Y, Sukumaran P, Quenum Zangbede F, Jondle C, Sharma A, et al. M1 Macrophage Polarization Is Dependent on TRPC1-Mediated Calcium Entry. iScience. 2018;8:85-102 pubmed publisher
	He X, Song S, Ayon R, Balisterieri A, Black S, Makino A, et al. Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells. Am J Physiol Cell Physiol. 2018;314:C504-C517 pubmed publisher
	Stagno M, Zacharopoulou N, Bochem J, Tsapara A, Pelzl L, Al Maghout T, et al. Istaroxime Inhibits Motility and Down-Regulates Orai1 Expression, SOCE and FAK Phosphorylation in Prostate Cancer Cells. Cell Physiol Biochem. 2017;42:1366-1376 pubmed publisher
	Mai X, Shang J, Liang S, Yu B, Yuan J, Lin Y, et al. Blockade of Orai1 Store-Operated Calcium Entry Protects against Renal Fibrosis. J Am Soc Nephrol. 2016;27:3063-3078 pubmed

image

image 1 :

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 2) and human HL-60 acute promyelocytic leukemia cell line lysate (lanes 3 and 4): - 1,3. Anti-Human Orai1 (extracellular) Antibody (#ACC-060), (1:200). 2,4. Anti-Human Orai1 (extracellular) Antibody, preincubated with Human Orai1 (extracellular) Blocking Peptide (#BLP-CC060).

image 2 :

Peptide (C)KKQPGQPRPTSK corresponding to amino acid residues 203-214 of human Orai1 (AccessionQ96D31). 2ndextracellular loop.

image 3 :

Cell surface detection of Orai1 in intact HL-60 (A)and Jurkat cells (B). - ___Unstained cells.___Cells +Anti-Human Orai1 (extracellular)Antibody (#ACC-060) (5-10 g antibody/0.5-1 x 106cells).

product information

CAT :

ACC-060

SKU :

ACC-060-CF_0.2 ml

Product Name :

Anti-Human Orai1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q96D31

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CC060

Homology :

Canis - identical; bovine - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1

Product Page - Scientific background :

Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Short Description :

A Rabbit Polyclonal Antibody to Human Orai1 Channel

Long Description :

Anti-Human Orai1 (extracellular) Antibody (#ACC-060) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize Orai1 from human samples only.

Negative Control :

BLP-CC060

Positive Control :

Synonyms :

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ORAI1

AB Specifictiy :

Will not recognize mouse or rat Orai1.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage