Anti-Human Orai1 (extracellular)-FITC Antibody | Alomone Labs ACC-060-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human Orai1 (extracellular)-FITC Antibody

catalog :

ACC-060-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Movsisyan N, Pardo L. Kv10.1 Regulates Microtubule Dynamics during Mitosis. Cancers (Basel). 2020;12: pubmed publisher

image

image 1 :

Cell surface detection of Orai1 in live Jurkat (human acute T cell leukaemia) cells: Cell surface detection of Orai1 in live Jurkat (human acute T cell leukaemia) cells: Black line: Unstained cells. Red line: Cells + Anti-Human Orai1 (extracellular)-FITC Antibody (#ACC-060-F), (10 µg/1x106 cells).

product information

CAT :

ACC-060-F

SKU :

ACC-060-F_50 mcl

Product Name :

Anti-Human Orai1 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q96D31

Applications :

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CC060

Homology :

Canis - identical; bovine - 12/13 amino acid residues

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1

Product Page - Scientific background :

Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9,10.

Applications may also work in :

Supplier :

Alomone Labs

Target :

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Short Description :

A Rabbit Polyclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-Human Orai1 (extracellular) Antibody (#ACC-060) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize Orai1 from human samples only. Anti-Human Orai1 (extracellular)-FITC Antibody (#ACC-060-F) is directly conjugated to FITC. This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ORAI1

AB Specifictiy :

Will not recognize mouse or rat Orai1.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-060), and direct flow cytometry analysis (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage