Anti-TRPM3 (extracellular) Antibody | Alomone Labs ACC-050 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPM3 (extracellular) Antibody

catalog :

ACC-050

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Eaton Fitch N, Cabanas H, du Preez S, Staines D, Marshall Gradisnik S. The effect of IL-2 stimulation and treatment of TRPM3 on channel co-localisation with PIP2 and NK cell function in myalgic encephalomyelitis/chronic fatigue syndrome patients. J Transl Med. 2021;19:306 pubmed publisher
Vangeel L, Benoit M, Miron Y, Miller P, De Clercq K, Chaltin P, et al. Functional expression and pharmacological modulation of TRPM3 in human sensory neurons. Br J Pharmacol. 2020;177:2683-2695 pubmed publisher
Papanikolaou M, Lewis A, Butt A. Store-operated calcium entry is essential for glial calcium signalling in CNS white matter. Brain Struct Funct. 2017;222:2993-3005 pubmed publisher
Suzuki A, Shinoda M, Honda K, Shirakawa T, Iwata K. Regulation of transient receptor potential vanilloid 1 expression in trigeminal ganglion neurons via methyl-CpG binding protein 2 signaling contributes tongue heat sensitivity and inflammatory hyperalgesia in mice. Mol Pain. 2016;12: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6): - 1-3. Anti-TRPM3 (extracellular) Antibody (#ACC-050), (1:200).4-6. Anti-TRPM3 (extracellular) Antibody, preincubated with TRPM3 (extracellular) Blocking Peptide (#BLP-CC050).

image 2 :

Expression of TRPM3 in mouse cerebellum. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TRPM3 (extracellular) Antibody (#ACC-050), (1:1200), followed by goat anti-rabbit-Alexa-488. A. TRPM3 immunoreactivity (green) appears in the purkinje cell layer (P, arrows) and in the molecular layer (MOL). B. Pre-incubation of the antibody with TRPM3 (extracellular) Blocking Peptide (#BLP-CC050), suppressed staining. Cell nuclei are stained with DAPI (blue).

image 3 :

Expression of Glucose Transporter 4 in rat olfactory bulb - Immunohistochemical staining of rat olfactory bulb free floating frozen sections using Anti-GLUT4-ATTO-594 Antibody (#AGT-024-AR) (1:40). GLUT4 immunoreactivity (red) is strongly detected in some glomeruli (vertical arrows). Lower immunoreactivity in other glomeruli (horizontal arrows) is also observed. Nuclei are stained using DAPI as the counterstain.

product information

CAT :

ACC-050

SKU :

ACC-050-CF_0.2 ml

Product Name :

Anti-TRPM3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9HCF6

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC050

Homology :

Rat, mouse - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

KNKDDMPYMSQAQEIH(C), corresponding to amino acid residues 816-831 of human TRPM3

Product Page - Scientific background :

Transient receptor potential (TRP) channels are relatively non-selective ion channels enabling the exchange of cations down their electrochemical gradient. This exchange enables the intracellular rise in Na+ and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction1. They are activated by an extremely broad range of stimuli namely, temperature, voltage, pH, endocrine factors as well as signaling molecules2. The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency3. In addition, TRP channels have in the C-terminal intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 - a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4.The TRPM subfamily consists of 8 members divided in to three major groups: TRPM1/3, TRPM4/5, and TRPM6/7. TRPM2 and TRPM8 are not included in any of the groups since they are quite different even with respect to each other. TRPM3 is alternatively spliced into active variants5,6. TRPM3a1 and TRPM3a2 in mouse are monovalent and divalent-selective channels, respectively. This differential selectivity strongly suggests that their cellular role depends on the relative expression of each variant type. In addition, both splice variants display constitutive outwardly rectifying currents. They somewhat resemble TRPM6 and TRPM7 channels such that their currents are inhibited by intracellular Mg2+ 1. TRPM3 is highly expressed in the kidney, and to a lesser extent in the brain, testis, and spinal cord7.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily M member 3, Long transient receptor potential channel 3, LTrpC3, Melastatin-2, MLSN2

Short Description :

A Rabbit Polyclonal Antibody to TRPM3 Channel

Long Description :

Anti-TRPM3 (extracellular) Antibody (#ACC-050) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPM3 from human, rat, and mouse samples.

Negative Control :

BLP-CC050

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily M member 3, Long transient receptor potential channel 3, LTrpC3, Melastatin-2, MLSN2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPM3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage