Anti-TRPM8 (extracellular) Antibody | Alomone Labs ACC-049 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPM8 (extracellular) Antibody

catalog :

ACC-049

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 18

Reference
Stratiievska A, Filippova O, xd6 zpolat T, Byrne D, Lawrence Bailey S, Mollica M, et al. Cold temperature induces a TRPM8-independent calcium release from the endoplasmic reticulum in human platelets. bioRxiv. 2023;: pubmed publisher
Genovesi S, Moro R, Vignoli B, De Felice D, Canossa M, Montironi R, et al. Trpm8 Expression in Human and Mouse Castration Resistant Prostate Adenocarcinoma Paves the Way for the Preclinical Development of TRPM8-Based Targeted Therapies. Biomolecules. 2022;12: pubmed publisher
Thapa D, Barrett B, Argunhan F, Brain S. Influence of Cold-TRP Receptors on Cold-Influenced Behaviour. Pharmaceuticals (Basel). 2021;15: pubmed publisher
Thapa D, Valente J, Barrett B, Smith M, Argunhan F, Lee S, et al. Dysfunctional TRPM8 signalling in the vascular response to environmental cold in ageing. elife. 2021;10: pubmed publisher
Lunardi A, Barbareschi M, Carbone F, Morelli L, Brunelli M, Fortuna N, et al. TRPM8 protein expression in hormone naïve local and lymph node metastatic prostate cancer. Pathologica. 2021;113:95-101 pubmed publisher
Henao J, Grismaldo A, Barreto A, Rodríguez Pardo V, Mejía Cruz C, Leal Garcia E, et al. TRPM8 Channel Promotes the Osteogenic Differentiation in Human Bone Marrow Mesenchymal Stem Cells. Front Cell Dev Biol. 2021;9:592946 pubmed publisher
Acharya T, Kumar S, Tiwari N, Ghosh A, Tiwari A, Pal S, et al. TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering. Sci Rep. 2021;11:3730 pubmed publisher
Huang Y, Li S, Jia Z, Zhao W, Zhou C, Zhang R, et al. Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy. Front Oncol. 2020;10:573127 pubmed publisher
Alaimo A, Lorenzoni M, Ambrosino P, Bertossi A, Bisio A, Macchia A, et al. Calcium cytotoxicity sensitizes prostate cancer cells to standard-of-care treatments for locally advanced tumors. Cell Death Dis. 2020;11:1039 pubmed publisher
Luo Q, Wang B, Chen X, Qiu H, Li W, Yan X, et al. Acute stress induces visceral hypersensitivity via glucocorticoid receptor-mediated membrane insertion of TRPM8: Involvement of a non-receptor tyrosine kinase Pyk2. Neurogastroenterol Motil. 2020;32:1514-1528 pubmed publisher
Zhang X. Direct Gαq Gating Is the Sole Mechanism for TRPM8 Inhibition Caused by Bradykinin Receptor Activation. Cell Rep. 2019;27:3672-3683.e4 pubmed publisher
Spencer K, Belgacem Y, Visina O, Shim S, Genus H, Borodinsky L. Growth at Cold Temperature Increases the Number of Motor Neurons to Optimize Locomotor Function. Curr Biol. 2019;29:1787-1799.e5 pubmed publisher
Hossain M, Ando H, Unno S, Masuda Y, Kitagawa J. Activation of TRPV1 and TRPM8 Channels in the Larynx and Associated Laryngopharyngeal Regions Facilitates the Swallowing Reflex. Int J Mol Sci. 2018;19: pubmed publisher
Hirai A, Aung N, Ohe R, Nishida A, Kato T, Meng H, et al. Expression of TRPM8 in human reactive lymphoid tissues and mature B-cell neoplasms. Oncol Lett. 2018;16:5930-5938 pubmed publisher
Naziroglu M, Blum W, Jósvay K, Ciğ B, Henzi T, Olah Z, et al. Menthol evokes Ca2+ signals and induces oxidative stress independently of the presence of TRPM8 (menthol) receptor in cancer cells. Redox Biol. 2018;14:439-449 pubmed publisher
Liu C, Su H, Wan H, Qin Q, Wu X, Kong X, et al. Forsythoside A exerts antipyretic effect on yeast-induced pyrexia mice via inhibiting transient receptor potential vanilloid 1 function. Int J Biol Sci. 2017;13:65-75 pubmed publisher
Gong K, Jasmin L. Sustained Morphine Administration Induces TRPM8-Dependent Cold Hyperalgesia. J Pain. 2017;18:212-221 pubmed publisher
Melanaphy D, Johnson C, Kustov M, Watson C, Borysova L, Burdyga T, et al. Ion channel mechanisms of rat tail artery contraction-relaxation by menthol involving, respectively, TRPM8 activation and L-type Ca2+ channel inhibition. Am J Physiol Heart Circ Physiol. 2016;311:H1416-H1430 pubmed publisher

image

image 1 :

Western blot analysis of prostate carcinoma cell lines DU145 (lanes 1 and 3), Human LNCaP prostate carcinoma (lanes 2 and 4) and mouse-TRPM8 transfected HEK-293 (lanes 5 and 6) cell lysates: - 1,2,5. Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:200).3,4,6. Anti-TRPM8 (extracellular) Antibody, preincubated with TRPM8 (extracellular) Blocking Peptide (#BLP-CC049).

image 2 :

Expression of TRPM8 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:100). TRPM8 is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.

image 3 :

Immuno-colocalization of TRPM8 and TrkA in rat DRG - Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sectionsusingAnti-TrkA (extracellular)-ATTO-633 Antibody(#ANT-018-FR) (1:60) andAnti-TRPM8 (extracellular)Antibody (#ACC-049) (1:300). A. TRPM8 labeling followed by goat-anti-rabbit-Alexa-488 (green). B. The same section was then labeled for TrkA (purple). C. Merge of A and B demonstrates co-localization of TRPM8 and TrkA in rat DRG (arrows). Cell nuclei were stained with DAPI (blue).

product information

CAT :

ACC-049

SKU :

ACC-049-CF_0.2 ml

Product Name :

Anti-TRPM8 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7Z2W7

Applications :

IC IF IFC IHC IP LCI N WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC049

Homology :

Canis - identical; bovine - 12/13 amino acid residues identical; rat, mouse - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

SDVDGTTYDFAHC, corresponding to amino acid residues 917-929 of human TRPM8

Product Page - Scientific background :

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first member, melastatin (TRPM1), whose gene was identified in metastatic and benign melanomas.1 The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family.1The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7.1The TRPM8 channel is the cold and menthol receptor activated by either cold temperatures(~28°C) or menthol.2TRPM8 is expressed in dorsal root ganglia (DRGs) where about 5%-10% of the small diameter DRG neurons express the channel.3,4 In DRGs, TRPM8 expressing neurons do not express the TRPV1 channel.5 Overexpression of TRPM8 was found in prostate cancer cells. However, the physiological and pathological roles that these cells play is still elusive.6 In prostate, it was suggested that TRPM8 might play a possible role in the progression of cancer to the metastatic stage.6

Applications may also work in :

IC IF IFC IHC IP LCI N WB

Supplier :

Alomone Labs

Target :

TRPP8, LTrpC6, Transient receptor potential cation channel subfamily M member 8

Short Description :

A Rabbit Polyclonal Antibody to TRPM8 Channel

Long Description :

Anti-TRPM8 (extracellular) Antibody (#ACC-049) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and neutralization applications. It has been designed to recognize TRPM8 from human, rat, and mouse samples.

Negative Control :

BLP-CC049

Positive Control :

Synonyms :

TRPP8, LTrpC6, Transient receptor potential cation channel subfamily M member 8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPM8

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:60-1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage