Anti-TRPM7 Antibody | Alomone Labs ACC-047 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPM7 Antibody

catalog :

ACC-047

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 10

Reference
Bai Z, Feng J, Franken G, Al Saadi N, Cai N, Yu A, et al. CNNM proteins selectively bind to the TRPM7 channel to stimulate divalent cation entry into cells. PLoS Biol. 2021;19:e3001496 pubmed publisher
Gwanyanya A, Andriulė I, Istrate B, Easmin F, Mubagwa K, Macianskiene R. Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Sensitive TRPM6 and TRPM7-like Current. Int J Mol Sci. 2021;22: pubmed publisher
Andriulė I, Pangonyte D, Almanaitytė M, Patamsyte V, Kuprytė M, Karciauskas D, et al. Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart. Sci Rep. 2021;11:15445 pubmed publisher
Jeong J, Lee S, Kho A, Hong D, Kang D, Kang B, et al. The Transient Receptor Potential Melastatin 7 (TRPM7) Inhibitors Suppress Seizure-Induced Neuron Death by Inhibiting Zinc Neurotoxicity. Int J Mol Sci. 2020;21: pubmed publisher
Sadowska A, Altinay B, Hitzl W, Ferguson S, Wuertz Kozak K. Hypo-Osmotic Loading Induces Expression of IL-6 in Nucleus Pulposus Cells of the Intervertebral Disc Independent of TRPV4 and TRPM7. Front Pharmacol. 2020;11:952 pubmed publisher
Yap J, Tai Y, Fröhlich J, Fong C, Yin J, Foo Z, et al. Ambient and supplemental magnetic fields promote myogenesis via a TRPC1-mitochondrial axis: evidence of a magnetic mitohormetic mechanism. FASEB J. 2019;33:12853-12872 pubmed publisher
Liu A, Wu J, Yang C, Wu Y, Zhang Y, Zhao F, et al. TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury. Sci Rep. 2018;8:5510 pubmed publisher
Beesetty P, Wieczerzak K, Gibson J, Kaitsuka T, Luu C, Matsushita M, et al. Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry. Sci Rep. 2018;8:3023 pubmed publisher
Ogunrinde A, Pereira R, Beaton N, Lam D, Whetstone C, Hill C. Hepatocellular differentiation status is characterized by distinct subnuclear localization and form of the chanzyme TRPM7. Differentiation. 2017;96:15-25 pubmed publisher
Macianskiene R, Almanaitytė M, Jekabsone A, Mubagwa K. Modulation of Human Cardiac TRPM7 Current by Extracellular Acidic pH Depends upon Extracellular Concentrations of Divalent Cations. PLoS ONE. 2017;12:e0170923 pubmed publisher

image

image 1 :

Western blot analysis of rat GH3 pituitary tumor cell line (lanes 1 and 2) and mouse brain (lanes 3 and 4) lysates: - 1,3. Anti-TRPM7 Antibody (#ACC-047), (1:200).2,4. Anti-TRPM7 Antibody, preincubated with TRPM7 Blocking Peptide (#BLP-CC047).

image 2 :

Expression of TRPM7 in rat pituitary gland - Immunohistochemical staining of rat hypophysis paraffin embedded transversal sections using Anti-TRPM7 Antibody (#ACC-047), (1:50). TRPM7 is stained in both the posterior part or neurohypophysis (N) and the anterior part or adenohypophysis (A). Note that stain is highly specific for andocrine cells in the adenohypophysis. Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the Counterstain.

image 3 :

Expression of TRPM7 in rat pituitary gland - Immunohistochemical staining of rat hypophysis paraffin embedded transversal sections using Anti-TRPM7Antibody (#ACC-047) (1:50). TRPM7 is stained in both the posterior part or neurohypophysis (N) and the anterior part or adenohypophysis (A). Note that stain is highly specific for andocrine cells in the adenohypophysis. Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the Counterstain.

product information

CAT :

ACC-047

SKU :

ACC-047-CF_0.2 ml

Product Name :

Anti-TRPM7 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q96QT4

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC047

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CKRRKKDKTSDGPKLFLTEE, corresponding to amino acid residues 1146-1165 of human TRPM7

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.1-4The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5TRPM7 and TRPM6 are involved in Mg2+ homeostasis and are unique among the TRP family members, possessing a functional kinase domain at their C-terminus.6,7 Although the kinase is not necessary to the function of the channel it may have a role in modulating the activation of channel 7.Recent work demonstrated that TRPM7 is a critical mediator of anoxic cell death.7,8

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

LTrpC7, TRP-PLIK, Channel-kinase 1, CHAK1, ALSPDC

Short Description :

A Rabbit Polyclonal Antibody to TRPM7 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of human TRPM7. Anti-TRPM7 Antibody (#ACC-047) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPM7 from human, rat, and mouse samples.

Negative Control :

BLP-CC047

Positive Control :

Synonyms :

LTrpC7, TRP-PLIK, Channel-kinase 1, CHAK1, ALSPDC

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPM7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage