Anti-TRPM6 (extracellular) Antibody | Alomone Labs ACC-046 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPM6 (extracellular) Antibody

catalog :

ACC-046

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Andriulė I, Pangonyte D, Almanaitytė M, Patamsyte V, Kuprytė M, Karciauskas D, et al. Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart. Sci Rep. 2021;11:15445 pubmed publisher
Chen B, Gao Y, Wei S, Low S, Ng G, Yu D, et al. TRPM4-specific blocking antibody attenuates reperfusion injury in a rat model of stroke. Pflugers Arch. 2019;471:1455-1466 pubmed publisher
Beesetty P, Wieczerzak K, Gibson J, Kaitsuka T, Luu C, Matsushita M, et al. Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry. Sci Rep. 2018;8:3023 pubmed publisher
Suzuki Y, Watanabe M, Saito C, Tominaga M. Expression of the TRPM6 in mouse placental trophoblasts; potential role in maternal-fetal calcium transport. J Physiol Sci. 2017;67:151-162 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 4), rat testis (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates: - 1-3. Anti-TRPM6 (extracellular) Antibody (#ACC-046), (1:200).4-6. Anti-TRPM6 (extracellular) Antibody, preincubated with TRPM6 (extracellular) Blocking Peptide (#BLP-CC046).

image 2 :

Expression of TRPM6 in rat hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM6 (extracellular) Antibody (#ACC-046), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region showed TRPM6 immunoreactivity (green) in in the pyramidal cell layer (P) and apical dendrites (arrows). B. Pre-incubation of the antibody with TRPM6 (extracellular) Blocking Peptide (BLP-CC046), suppressed staining. Cell nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 12. Anti-ASTN1 (extracellular) Antibody (#ANR-151) (1:200).34. Anti-ASTN1 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-046

SKU :

ACC-046-CF_0.2 ml

Product Name :

Anti-TRPM6 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8CIR4

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC046

Homology :

Rat - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CVKDYDLERGPDEK, corresponding to amino acid residues 802-815 of mouse TRPM6

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.1-4The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, CHAK2, Melastatin-related TRP cation channel 6

Short Description :

A Rabbit Polyclonal Antibody to TRPM6 Channel

Long Description :

Anti-TRPM6 (extracellular) Antibody (#ACC-046) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPM6 from mouse, rat, and human samples.

Negative Control :

BLP-CC046

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, CHAK2, Melastatin-related TRP cation channel 6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPM6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage