Anti-TRPM5 Antibody | Alomone Labs ACC-045 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPM5 Antibody

catalog :

ACC-045

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:100	Goss G, Chaudhari N, Hare J, Nwojo R, Seidler B, Saur D, et al. Differentiation potential of individual olfactory c-Kit+ progenitors determined via multicolor lineage tracing. Dev Neurobiol. 2016;76:241-51 pubmed publisher
	Bos R, Drouillas B, Bouhadfane M, Pecchi E, Trouplin V, Korogod S, et al. Trpm5 channels encode bistability of spinal motoneurons and ensure motor control of hindlimbs in mice. Nat Commun. 2021;12:6815 pubmed publisher
	Wang C, Maeda M, Chen J, Wang M, Naruse K, Takahashi K. Production of TRPM4 knockout cell line using rat cardiomyocyte H9c2. MethodsX. 2021;8:101404 pubmed publisher
	Nomura K, Nakanishi M, Ishidate F, Iwata K, Taruno A. All-Electrical Ca2+-Independent Signal Transduction Mediates Attractive Sodium Taste in Taste Buds. Neuron. 2020;106:816-829.e6 pubmed publisher
	Chen B, Gao Y, Wei S, Low S, Ng G, Yu D, et al. TRPM4-specific blocking antibody attenuates reperfusion injury in a rat model of stroke. Pflugers Arch. 2019;471:1455-1466 pubmed publisher
	Yamaguchi S, Tanimoto A, Iwasa S, Otsuguro K. TRPM4 and TRPM5 Channels Share Crucial Amino Acid Residues for Ca2+ Sensitivity but Not Significance of PI(4,5)P2. Int J Mol Sci. 2019;20: pubmed publisher
	Kashio M, Wei Qi G, Ohsaki Y, Kido M, Taruno A. CALHM1/CALHM3 channel is intrinsically sorted to the basolateral membrane of epithelial cells including taste cells. Sci Rep. 2019;9:2681 pubmed publisher
	Yamashita J, Ohmoto M, Yamaguchi T, Matsumoto I, Hirota J. Skn-1a/Pou2f3 functions as a master regulator to generate Trpm5-expressing chemosensory cells in mice. PLoS ONE. 2017;12:e0189340 pubmed publisher
	Maeda T, Suzuki A, Koga K, Miyamoto C, Maehata Y, Ozawa S, et al. TRPM5 mediates acidic extracellular pH signaling and TRPM5 inhibition reduces spontaneous metastasis in mouse B16-BL6 melanoma cells. Oncotarget. 2017;8:78312-78326 pubmed publisher
	Ohmoto M, Ren W, Nishiguchi Y, Hirota J, Jiang P, Matsumoto I. Genetic Lineage Tracing in Taste Tissues Using Sox2-CreERT2 Strain. Chem Senses. 2017;42:547-552 pubmed publisher
	Nishiguchi Y, Ohmoto M, Koki J, Enomoto T, Kominami R, Matsumoto I, et al. Bcl11b/Ctip2 is required for development of lingual papillae in mice. Dev Biol. 2016;416:98-110 pubmed publisher

image

image 1 :

Western blot analysis of rat lung membrane (lanes 1 and 2), rat testes (lanes 3 and 4), mouse ms1 pancreas cells (lanes 5 and 7) and human LNCaP prostate cell (lanes 6 and 8) lysates: - 1,3,5,6. Anti-TRPM5 Antibody (#ACC-045), (1:200).2,4,7,8. Anti-TRPM5 Antibody, preincubated with TRPM5 Blocking Peptide (#BLP-CC045).

image 2 :

Expression of TRPM5 in rat tongue - Immunohistochemical staining of rat tongue paraffin embedded sections using Anti-TRPM5 Antibody (#ACC-045), (1:100). TRPM5 is expressed in discrete groups of cells deep in the sulcus of the papillae. Hematoxilin is used as the counterstain.

image 3 :

Expression of TRPM5 in rat tongue - Immunohistochemical staining of rat tongue paraffin embedded sections using Anti-TRPM5Antibody (#ACC-045) (1:100). TRPM5 is expressed in discrete groups of cells deep in the sulcus of the papillae. Hematoxilin is used as the counterstain.

product information

CAT :

ACC-045

SKU :

ACC-045-CF_0.2 ml

Product Name :

Anti-TRPM5 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JJH7

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC045

Homology :

Rat, human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

GSGKKRGKFVKVPS(C), corresponding to amino acid residues 32-45 of mouse TRPM5

Product Page - Scientific background :

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3. The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3. TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca2+. TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca2+ concentration4,5.TRPM5 is primarily expressed in taste buds and also in stomach, small intestine, uterus and testes2. Regulation of TRPM5 by G-protein-coupled receptors and PLC-mediated IP3 production has been substantiated by the colocalization of TRPM5, of a taste-cell-specific PLC isoform and of receptors for sweet, bitter and amino acid (umami) tastes in taste tissue. It has furthermore been shown that desensitization of TRPM5 following receptor stimulation can be reversed by phosphatidylinositol (4,5)-bisphosphate (PIP2), the substrate of PLC2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

LTrpC5, MTR1, Transient receptor potential cation channel subfamily M member 5

Short Description :

A Rabbit Polyclonal Antibody to TRPM5 Channel

Long Description :

Anti-TRPM5 Antibody (#ACC-045) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPM5 from mouse, rat and human samples.

Negative Control :

BLP-CC045

Positive Control :

Synonyms :

LTrpC5, MTR1, Transient receptor potential cation channel subfamily M member 5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPM5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage