Anti-TRPM4 Antibody | Alomone Labs ACC-044 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPM4 Antibody

catalog :

ACC-044

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 11

Reference
Fearey B, Binkle L, Mensching D, Schulze C, Lohr C, Friese M, et al. A glibenclamide-sensitive TRPM4-mediated component of CA1 excitatory postsynaptic potentials appears in experimental autoimmune encephalomyelitis. Sci Rep. 2022;12:6000 pubmed publisher
Yoo S, Mittelstein D, Hurt R, Lacroix J, Shapiro M. Focused ultrasound excites cortical neurons via mechanosensitive calcium accumulation and ion channel amplification. Nat Commun. 2022;13:493 pubmed publisher
Wang C, Maeda M, Chen J, Wang M, Naruse K, Takahashi K. Production of TRPM4 knockout cell line using rat cardiomyocyte H9c2. MethodsX. 2021;8:101404 pubmed publisher
Guo Y, Yu Z, Wu J, Gong H, Kesteven S, Iismaa S, et al. The Ca2+-activated cation channel TRPM4 is a positive regulator of pressure overload-induced cardiac hypertrophy. elife. 2021;10: pubmed publisher
Cai Y, Zhang B, Yu M, Yang Y, Ao X, Zhu D, et al. Cholesterol Stimulates the Transient Receptor Potential Melastatin 4 Channel in mpkCCDc14 Cells. Front Pharmacol. 2021;12:627875 pubmed publisher
Masoli S, Tognolina M, Laforenza U, Moccia F, D Angelo E. Parameter tuning differentiates granule cell subtypes enriching transmission properties at the cerebellum input stage. Commun Biol. 2020;3:222 pubmed publisher
Kullmann F, Beckel J, McDonnell B, Gauthier C, Lynn A, Wolf Johnston A, et al. Involvement of TRPM4 in detrusor overactivity following spinal cord transection in mice. Naunyn Schmiedebergs Arch Pharmacol. 2018;391:1191-1202 pubmed publisher
Riquelme D, Silva I, Philp A, Huidobro Toro J, Cerda O, Trimmer J, et al. Subcellular Localization and Activity of TRPM4 in Medial Prefrontal Cortex Layer 2/3. Front Cell Neurosci. 2018;12:12 pubmed publisher
Leiva Salcedo E, Riquelme D, Cerda O, Stutzin A. TRPM4 activation by chemically- and oxygen deprivation-induced ischemia and reperfusion triggers neuronal death. Channels (Austin). 2017;11:624-635 pubmed publisher
Wu M, Zhai Y, Li Y, Hu Q, Wang Z, Wei S, et al. Hydrogen peroxide suppresses TRPM4 trafficking to the apical membrane in mouse cortical collecting duct principal cells. Am J Physiol Renal Physiol. 2016;311:F1360-F1368 pubmed publisher
Hristov K, Smith A, Parajuli S, Malysz J, Rovner E, Petkov G. Novel regulatory mechanism in human urinary bladder: central role of transient receptor potential melastatin 4 channels in detrusor smooth muscle function. Am J Physiol Cell Physiol. 2016;310:C600-11 pubmed publisher

image

image 1 :

Western blot analysis of rat lung (lanes 1 and 5), rat heart membrane (lanes 2 and 6), mouse brain membrane (lanes 3 and 7) and ms1 mouse pancreas cells lysate (lanes 4 and 8): - 1-4. Anti-TRPM4 Antibody (#ACC-044), (1:200).5-8. Anti-TRPM4 Antibody, preincubated with TRPM4 Blocking Peptide (#BLP-CC044).

image 2 :

Expression of TRPM4 in rat lung - Immunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM4 Antibody (#ACC-044), (1:100). TRPM4 is expressed in the respiratory epithelium of the bronchioli (arrows). Hematoxilin is used as the counterstain.

image 3 :

Expression of TRPM4 in rat lung - Immunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM4Antibody (#ACC-044) (1:100). TRPM4 is expressed in the respiratory epithelium of the bronchioli (arrows). Hematoxilin is used as the counterstain.

product information

CAT :

ACC-044

SKU :

ACC-044-CF_0.2 ml

Product Name :

Anti-TRPM4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8TD43

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC044

Homology :

Rat, mouse - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

EKEQSWIPKIFKK(C), corresponding to amino acid residues 5-17 of human TRPM4

Product Page - Scientific background :

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3. The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3. TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca2+. TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca2+ concentration4.TRPM4 is expressed at relatively high levels in cardiac tissues.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Melastatin-4, LTrpC4, Calcium-activated non-selective cation channel 1

Short Description :

A Rabbit Polyclonal Antibody To TRPM4 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human TRPM4. Anti-TRPM4 Antibody (#ACC-044) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPM4 from human, rat, and mouse samples.

Negative Control :

BLP-CC044

Positive Control :

Synonyms :

Melastatin-4, LTrpC4, Calcium-activated non-selective cation channel 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPM4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage