Anti-TRPM2 Antibody | Alomone Labs ACC-043 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPM2 Antibody

catalog :

ACC-043

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Cheng L, Luo Q, Chen S. Expression of TRP Channels in Colonic Mucosa of IBS-D Patients and Its Correlation with the Severity of the Disease. Gastroenterol Res Pract. 2022;2022:7294775 pubmed publisher
Meléndez García R, Arredondo Zamarripa D, Arnold E, Ruiz Herrera X, Noguez Imm R, Baeza Cruz G, et al. Prolactin protects retinal pigment epithelium by inhibiting sirtuin 2-dependent cell death. EBioMedicine. 2016;7:35-49 pubmed publisher

image

image 1 :

Western blot analysis of rat lung membrane (lanes 1 and 4), rat brain membrane (lanes 2 and 5) and human K562 chronic myelogeneous cells (lanes 3 and 6) lysates: - 1-3. Anti-TRPM2 Antibody (#ACC-043), (1:200).4-6. Anti-TRPM2 Antibody, preincubated with TRPM2 Blocking Peptide (#BLP-CC043).

image 2 :

Expression of TRPM2 in rat lung - Immunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM2 Antibody (#ACC-043), (1:100). TRPM2 is expressed in the respiratory epithelium (black arrows). In addition, staining is also present in smooth muscle, both bronchiolar (arrowheads) and vascular (red arrows). Hematoxilin is used as the counterstain.

image 3 :

Expression of TRPM2 in rat lung - Immunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM2Antibody (#ACC-043) (1:100). TRPM2 is expressed in the respiratory epithelium (black arrows). In addition staining is also present in smooth muscle both bronchiolar (arrowheads) and vascular (red arrows). Hematoxilin is used as the counterstain.

product information

CAT :

ACC-043

SKU :

ACC-043-CF_0.2 ml

Product Name :

Anti-TRPM2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q91YD4

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC043

Homology :

Rat - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)HTFQGKEWDPKKHVQE, corresponding to amino acid residues 105-120 of mouse TRPM2

Product Page - Scientific background :

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3. The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3. The C-terminal regions of three TRPM members (TRPM2, TRPM6 and TRPM7) are occupied by enzymatic domains. Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates2,4.TRPM2 (LTRpC2, TrpC7) is a Ca2+-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified5,3.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily M member 2, LTrpC2, TrpC7

Short Description :

A Rabbit Polyclonal Antibody to TRPM2 Channel

Long Description :

Anti-TRPM2 Antibody (#ACC-043) is a highly specific antibody directed against an intracellular epitope of mouse TRPM2. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPM2 from mouse, rat, and human samples.

Negative Control :

BLP-CC043

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily M member 2, LTrpC2, TrpC7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPM2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage