Anti-TRPM2-ATTO Fluor-594 Antibody | Alomone Labs ACC-043-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPM2-ATTO Fluor-594 Antibody

catalog :

ACC-043-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Fanczal J, Pallagi P, Görög M, Diszházi G, Almassy J, Madácsy T, et al. TRPM2-mediated extracellular Ca2+ entry promotes acinar cell necrosis in biliary acute pancreatitis. J Physiol. 2020;598:1253-1270 pubmed publisher

image

image 1 :

Expression of TRPM2 in rat lung - Immunohistochemical staining of rat lung frozen sections using Anti-TRPM2-ATTO Fluor-594 Antibody (#ACC-043-AR), (1:50). A. TRPM2 staining is specific for respiratory epithelium in the bronchi. B. Merged with bright field.

image 2 :

Expression of TRPM2 in rat lung - Immunohistochemical staining of rat lung frozen sections using Anti-TRPM2-ATTO-594Antibody (#ACC-043-AR) (1:50). A. TRPM2 staining is specific for respiratory epithelium in the bronchi. B. Merged with bright field.

image 3 :

Cell surface detection of S1PR3 in live intact mouse J774 macrophage cells: - ___Cells.___Cells + rabbit IgG isotype control-FITC.___Cells +Anti-S1PR3 (EDG3) (extracellular)-FITC Antibody (#ASR-013-F) (2.5 g).

product information

CAT :

ACC-043-AR

SKU :

ACC-043-AR_50 mcl

Product Name :

Anti-TRPM2-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q91YD4

Applications :

IC IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC043

Homology :

Rat - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, N-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)HTFQGKEWDPKKHVQE, corresponding to amino acid residues 105-120 of mouse TRPM2

Product Page - Scientific background :

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3.The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3.The C-terminal regions of three TRPM members (TRPM2, TRPM6 and TRPM7) are occupied by enzymatic domains. Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates2,4.TRPM2 is a Ca2+-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified5,3.

Applications may also work in :

IC IF IHC

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily M member 2, LTrpC2, TrpC7

Short Description :

A Rabbit Polyclonal Antibody to TRPM2 Channel Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-TRPM2 Antibody (#ACC-043) is a highly specific antibody directed against an intracellular epitope of mouse TRPM2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPM2 from mouse, rat, and human samples. Anti-TRPM2-ATTO Fluor-594 Antibody (#ACC-043-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-TRPM2-ATTO Fluor-594 Antibody can be used for immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-CC043

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily M member 2, LTrpC2, TrpC7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPM2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-043), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage