Anti-Human TRPM1 Antibody | Alomone Labs ACC-041 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human TRPM1 Antibody

catalog :

ACC-041

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Do H, Ohbuchi T, Yokoyama M, Kitamura T, Wakasugi T, Ohkubo J, et al. Decreased ciliary beat responsiveness to acetylcholine in the nasal polyp epithelium. Clin Otolaryngol. 2019;44:356-365 pubmed publisher

image

image 1 :

Western blot analysis of A875 (human melanoma cell line) lysate: - 1. Anti-Human TRPM1 Antibody (#ACC-041), (1:200).2. Anti-Human TRPM1 Antibody, preincubated with Human TRPM1 Blocking Peptide (#BLP-CC041).

image 2 :

Peptide (C)EEKKVKKEKASTETE corresponding to amino acid residues 1518-1533 of human TRPM1 (Accession O75560). Intracellular C-terminus.

image 3 :

Expression of CHERP in mouse brain - Immunohistochemical staining of mouse cortex and hippocampus using Anti-CHERP-ATTO-594 Antibody (#ACC-330-AR). A. CHERP staining (red) in cortex appears in in cortical neurons (arrows). B. In hippocampus CHERP staining (red) appears in neurons of pyramidal layer (P) in CA3 region. Nuclei are stained using DAPI (blue).

product information

CAT :

ACC-041

SKU :

ACC-041-CF_0.2 ml

Product Name :

Anti-Human TRPM1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O75560

Applications :

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CC041

Homology :

Canis - 12/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EEKKVKKEKASTETE, corresponding to amino acid residues 1518-1533 of human TRPM1

Product Page - Scientific background :

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas. The TRPM family consist of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7.The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family. TRPM channels were suggested to play a role in tumourigenesis, proliferation and differentiation.Recently, TRPM1 was found to be down-regulated in a highly metastatic line. The loss of TRPM1 expression has been reported to be correlated to melanoma aggressiveness.Two alternative splice variants of TRPM1 were reported; short (TRPM1-S) and long (TRPM1-L). The TRPM1-S was described to be expressed at human melanocyte and mainly in metastasic melanoma cell lines. While TRPM1-L is expressed at murine CNS.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Melastatin-1, MLSN1, LTrpC1, Transient receptor potential cation channel subfamily M member 1

Short Description :

A Rabbit Polyclonal Antibody to Human TRPM1 Channel

Long Description :

Anti-Human TRPM1 Antibody (#ACC-041) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPM1 from human samples only.

Negative Control :

BLP-CC041

Positive Control :

Synonyms :

Melastatin-1, MLSN1, LTrpC1, Transient receptor potential cation channel subfamily M member 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPM1

AB Specifictiy :

Will not recognize rat or mouse TRPM1.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage