Anti-TRPV2 (VRL1) (extracellular) Antibody | Alomone Labs ACC-039 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPV2 (VRL1) (extracellular) Antibody

catalog :

ACC-039

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Yadav M, Goswami C. TRPV2 interacts with actin and reorganizes submembranous actin cytoskeleton. Biosci Rep. 2020;40: pubmed publisher
Chapotte Baldacci C, Lizot G, Jajkiewicz C, Lévêque M, Penna A, Magaud C, et al. Fine Tuning of Calcium Constitutive Entry by Optogenetically-Controlled Membrane Polarization: Impact on Cell Migration. Cells. 2020;9: pubmed publisher
Jansen C, Shimoda L, Starkus J, Lange I, Rysavy N, Maaetoft Udsen K, et al. In vitro exposure to Hymenoptera venom and constituents activates discrete ionotropic pathways in mast cells. Channels (Austin). 2019;13:264-286 pubmed publisher
Huang R, Wang F, Yang Y, Ma W, Lin Z, Cheng N, et al. Recurrent activations of transient receptor potential vanilloid-1 and vanilloid-4 promote cellular proliferation and migration in esophageal squamous cell carcinoma cells. FEBS Open Bio. 2019;9:206-225 pubmed publisher
Zhang H, Xiao J, Hu Z, Xie M, Wang W, He D. Blocking transient receptor potential vanilloid 2 channel in astrocytes enhances astrocyte-mediated neuroprotection after oxygen-glucose deprivation and reoxygenation. Eur J Neurosci. 2016;44:2493-2503 pubmed publisher

image

image 1 :

Western blot analysis of ND7/23 cell line membrane (lanes 1 and 3), RBL lysates (lanes 2 and 4) and rat brain membrane (lanes 5and 6): - 1,2,5. Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039), (1:200).3,4,6. Anti-TRPV2 (VRL1) (extracellular) Antibody, preincubated with TRPV2/VRL1 (extracellular) Blocking Peptide (#BLP-CC039).

image 2 :

Expression of TRPV2 in mouse DRG - Immunohistochemical staining of TRPV2 in mouse dorsal root ganglion (DRG) using Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039). A. TRPV2 (green) appears in patches along the perimeter of the DRG (arrows). B. Neurons containing neurofilament 200 (red) are scattered in the DRG, also in patches (arrows). C. A merge of the two panels shows that the spatial distribution of neurofilament 200 and TRPV2 expression overlaps. However, DRGs showing robust expression of neurofilament 200 do not contain TRPV2.

image 3 :

Western blot analysis of ND7/23 cell line membrane (lanes 1 and 3) RBL lysates (lanes 2 and 4) and rat brain membrane (lanes 5 6): - 125.Anti-TRPV2 (VRL1) (extracellular)Antibody (#ACC-039) (1:200).346. Anti-TRPV2 (VRL1) (extracellular) Antibody preincubated with the control peptide antigen.

product information

CAT :

ACC-039

SKU :

ACC-039-CF_0.2 ml

Product Name :

Anti-TRPV2 (VRL1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WUD2

Applications :

IC IF IFC IHC IP LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC039

Homology :

Mouse - 15/16 amino acid residues identicalThe antibody will not recognize human TRPV2

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-termini of all TRPs are intracellular3.According to IUPHAR, the TRP family is comprised of numerous subfamilies on the basis of sequence homology; TRPC, TRPM, TRPV, TRPA, TRPML, and TRPP1-4. The TRPV subfamily consists of six members, TRPV1-65.Four members of the TRPV family have been described as thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (52°C) 6,7. Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation8-10. TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway8. The TRPV2 channel is expressed in DRG neurons, different brain regions and non-neuronal tissues such as the spleen, lung and intestine and in components of the immune system5,11,12.

Applications may also work in :

IC IF IFC IHC IP LCI WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B

Short Description :

A Rabbit Polyclonal Antibody to TRPV2 (VRL1) Channel

Long Description :

Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize TRPV2 from mouse and rat samples. The antibody will not recognize human TRPV2.

Negative Control :

BLP-CC039

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage