Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody | Alomone Labs ACC-039-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody

catalog :

ACC-039-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and rat RBL basophilic leukemia cell lysate (lanes 2 and 4): - 1,2. Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039-GP), (1:2000).3,4. Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody, preincubated with TRPV2/VRL1 (extracellular) Blocking Peptide (#BLP-CC039).

image 2 :

Multiplex staining of TRPV2 and mGluR5 in rat DRG - Immunohistochemistry of rat dorsal root ganglion using Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039-GP) (1:60) and Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR), (red), (1:60). A. TRPV2 staining (green). B. mGluR5 staining (red). C. Merge of A and B demonstrates co-localization of TRPV2 and mGluR5 in DRG cells. Nuclei are stained using DAPI as the counterstain (blue).

product information

CAT :

ACC-039-GP

SKU :

ACC-039-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WUD2

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-CC039

Homology :

Mouse - 15/16Human - will not recognize the human epitope

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-termini of all TRPs are intracellular3.According to IUPHAR, the TRP family is comprised of numerous subfamilies on the basis of sequence homology; TRPC, TRPM, TRPV, TRPA, TRPML, and TRPP1-4. The TRPV subfamily consists of six members, TRPV1-65.Four members of the TRPV family have been described as thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (52°C) 6,7. Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation8-10. TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway8. The TRPV2 channel is expressed in DRG neurons, different brain regions and non-neuronal tissues such as the spleen, lung and intestine and in components of the immune system5,11,12.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B

Short Description :

A Guinea Pig Polyclonal Antibody to TRPV2 (VRL1) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat TRPV2. Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039-GP, formerly AGP-033) raised in guinea pigs, can be used in western blot and immunocytochemistry. It has been designed to recognize TRPV2 from mouse and rat samples. The antigen used to immunize guinea pigs is the same as Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-CC039

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV2

AB Specifictiy :

Will not recognize the human epitope.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:60

Application Dilutions: Western blot wb :

1:2000

more info or order :

Alomone Labs product webpage