Anti-TRPA1 (extracellular) Antibody | Alomone Labs ACC-037 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPA1 (extracellular) Antibody

catalog :

ACC-037

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 27

Reference
Cheng L, Luo Q, Chen S. Expression of TRP Channels in Colonic Mucosa of IBS-D Patients and Its Correlation with the Severity of the Disease. Gastroenterol Res Pract. 2022;2022:7294775 pubmed publisher
Hossain M, Ando H, Unno S, Kitagawa J. TRPA1s act as chemosensors but not as cold sensors or mechanosensors to trigger the swallowing reflex in rats. Sci Rep. 2022;12:3431 pubmed publisher
Zhan Y, Wen Y, Zhang L, Shen X, Chen X, Wu X, et al. Paeoniflorin Improved Constipation in the Loperamide-Induced Rat Model via TGR5/TRPA1 Signaling-Mediated 5-Hydroxytryptamine Secretion. Evid Based Complement Alternat Med. 2021;2021:6076293 pubmed publisher
De Logu F, Souza Monteiro de Araújo D, Ugolini F, Iannone L, Vannucchi M, Portelli F, et al. The TRPA1 Channel Amplifies the Oxidative Stress Signal in Melanoma. Cells. 2021;10: pubmed publisher
Nie L, Jiang L, Quinn J, Grubb B, Wang M. TRPA1-Mediated Src Family Kinases Activity Facilitates Cortical Spreading Depression Susceptibility and Trigeminovascular System Sensitization. Int J Mol Sci. 2021;22: pubmed publisher
Oguri G, Nakajima T, Kikuchi H, Obi S, Nakamura F, Komuro I. Allyl isothiocyanate (AITC) activates nonselective cation currents in human cardiac fibroblasts: possible involvement of TRPA1. Heliyon. 2021;7:e05816 pubmed publisher
Leo M, Schmitt L, K xfc sterarent P, Kutritz A, Rassaf T, Kleinschnitz C, et al. Platinum-Based Drugs Cause Mitochondrial Dysfunction in Cultured Dorsal Root Ganglion Neurons. Int J Mol Sci. 2020;21: pubmed publisher
De Logu F, Ugolini F, Caporalini C, Palomba A, Simi S, Portelli F, et al. TRPA1 Expression in Synovial Sarcoma May Support Neural Origin. Biomolecules. 2020;10: pubmed publisher
Souza Monteiro de Araújo D, De Logu F, Adembri C, Rizzo S, Janal M, Landini L, et al. TRPA1 mediates damage of the retina induced by ischemia and reperfusion in mice. Cell Death Dis. 2020;11:633 pubmed publisher
Shin S, Itson Zoske B, Cai Y, Qiu C, Pan B, Stucky C, et al. Satellite glial cells in sensory ganglia express functional transient receptor potential ankyrin 1 that is sensitized in neuropathic and inflammatory pain. Mol Pain. 2020;16:1744806920925425 pubmed publisher
Jin L, Jagatheesan G, Lynch J, Guo L, Conklin D. Crotonaldehyde-induced vascular relaxation and toxicity: Role of endothelium and transient receptor potential ankyrin-1 (TRPA1). Toxicol Appl Pharmacol. 2020;398:115012 pubmed publisher
Goodwin G, Bove G, Dayment B, Dilley A. Characterizing the Mechanical Properties of Ectopic Axonal Receptive Fields in Inflamed Nerves and Following Axonal Transport Disruption. Neuroscience. 2020;429:10-22 pubmed publisher
Virk H, Rekas M, Biddle M, Wright A, Sousa J, Weston C, et al. Validation of antibodies for the specific detection of human TRPA1. Sci Rep. 2019;9:18500 pubmed publisher
Sahoo S, Majhi R, Tiwari A, Acharya T, Kumar P, Saha S, et al. Transient receptor potential ankyrin1 channel is endogenously expressed in T cells and is involved in immune functions. Biosci Rep. 2019;39: pubmed publisher
Bernardini M, Brossa A, Chinigo G, Grolez G, Trimaglio G, Allart L, et al. Transient Receptor Potential Channel Expression Signatures in Tumor-Derived Endothelial Cells: Functional Roles in Prostate Cancer Angiogenesis. Cancers (Basel). 2019;11: pubmed publisher
Cheng R, Feng Y, Liu D, Wang Z, Zhang J, Chen L, et al. The role of Nav1.7 and methylglyoxal-mediated activation of TRPA1 in itch and hypoalgesia in a murine model of type 1 diabetes. Theranostics. 2019;9:4287-4307 pubmed publisher
Yang Y, Wang S, Kobayashi K, Hao Y, Kanda H, Kondo T, et al. TRPA1-expressing lamina propria mesenchymal cells regulate colonic motility. JCI Insight. 2019;4: pubmed publisher
Cun Jin S, Jian Hao X, Xu L, Feng Lun Z, Jie P, Ai Ming S, et al. X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation. Mol Pain. 2019;15:1744806919849201 pubmed publisher
Zheng X, Tai Y, He D, Liu B, Wang C, Shao X, et al. ETAR and protein kinase A pathway mediate ET-1 sensitization of TRPA1 channel: A molecular mechanism of ET-1-induced mechanical hyperalgesia. Mol Pain. 2019;15:1744806919842473 pubmed publisher
Jin L, Jagatheesan G, Guo L, Nystoriak M, Malovichko M, Lorkiewicz P, et al. Formaldehyde Induces Mesenteric Artery Relaxation via a Sensitive Transient Receptor Potential Ankyrin-1 (TRPA1) and Endothelium-Dependent Mechanism: Potential Role in Postprandial Hyperemia. Front Physiol. 2019;10:277 pubmed publisher
Jiang L, Ma D, Grubb B, Wang M. ROS/TRPA1/CGRP signaling mediates cortical spreading depression. J Headache Pain. 2019;20:25 pubmed publisher
CONKLIN D, Guo Y, Nystoriak M, Jagatheesan G, Obal D, Kilfoil P, et al. TRPA1 channel contributes to myocardial ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2019;316:H889-H899 pubmed publisher
Xu Y, Huang C, Deng H, Jia J, Wu Y, Yang J, et al. TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model. Turk J Gastroenterol. 2018;29:692-700 pubmed publisher
Pires P, Earley S. Neuroprotective effects of TRPA1 channels in the cerebral endothelium following ischemic stroke. elife. 2018;7: pubmed publisher
Han Q, Liu D, Convertino M, Wang Z, Jiang C, Kim Y, et al. miRNA-711 Binds and Activates TRPA1 Extracellularly to Evoke Acute and Chronic Pruritus. Neuron. 2018;99:449-463.e6 pubmed publisher
Berrout J, Kyriakopoulou E, Moparthi L, Hogea A, Berrout L, Ivan C, et al. TRPA1-FGFR2 binding event is a regulatory oncogenic driver modulated by miRNA-142-3p. Nat Commun. 2017;8:947 pubmed publisher
Liu C, Su H, Wan H, Qin Q, Wu X, Kong X, et al. Forsythoside A exerts antipyretic effect on yeast-induced pyrexia mice via inhibiting transient receptor potential vanilloid 1 function. Int J Biol Sci. 2017;13:65-75 pubmed publisher

image

image 1 :

Western blot analysis of rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates: - 1,3,4. Anti-TRPA1 (extracellular) Antibody (#ACC-037), (1:200).2,5,6. Anti-TRPA1 (extracellular) Antibody, preincubated with TRPA1 (extracellular) Blocking Peptide (#BLP-CC037).

image 2 :

Expression of TRPA1 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPA1 (extracellular) Antibody (#ACC-037) (1:200). A. TRPA1 staining (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.

image 3 :

Expression of TRPA1 in rat PC12 cells - Cell surface detection ofTRPA1 in intact living rat PC12 cells. A. Extracellular staining of cells usingAnti-TRPA1 (extracellular)Antibody (#ACC-037) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody(red). B. Live view of the cells. C. Merged image of A and B.

product information

CAT :

ACC-037

SKU :

ACC-037-CF_0.2 ml

Product Name :

Anti-TRPA1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O75762

Applications :

IC IF IFC IHC IP LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC037

Homology :

Bovine - identical; canis - 13/14 amino acid residues identical; rat - 12/14 amino acid residues identical; mouse - 9/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)NSTGIINETSDHSE, corresponding to amino acid residues 747-760 of human TRPA1

Product Page - Scientific background :

The TRPA family is comprised of only one mammalian member, the TRPA1 (formerly named ANKTM1). TRPA1 is expressed in peripheral sensory neurons, where it is suggested to contribute to the detection of painful stimuli.1Originally, it was thought that TRPA channels sensed painfully cold temperatures,2 but a more conservative description is that TRPA1 is sensitive to membrane/cytoskeletal perturbations caused by low temperatures3-5 and perhaps stretch.6 In addition, it is sensitive to pungent natural compounds present in cinnamon oil, mustard oil, and wintergreen oil.TRPA1 is also expressed in hair cells, where its role in sensing mechanical forces is still unclear and controversial.1TRPA1 has a similar structure to all other TRP ion channels; six transmembrane domains, intracellular N-and C-terminus. However, the N-terminal domain possesses 17 ankyrin repeats that might indicate its potential role as a mechanosensor.6,7In addition, TRPA1 is expressed in nociceptive neurons expressing TRPV1 and might serve as a marker for polymodal nociceptors.8

Applications may also work in :

IC IF IFC IHC IP LCI WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily A member 1, ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120

Short Description :

A Rabbit Polyclonal Antibody to TRPA1 Channel

Long Description :

Anti-TRPA1 (extracellular) Antibody (#ACC-037) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation immunohistochemistry, and live cell imaging applications. It has been designed to recognize TRPA1 from mouse, rat, and human samples.

Negative Control :

BLP-CC037

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily A member 1, ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage