Anti-TRPA1 (extracellular)-FITC Antibody | Alomone Labs ACC-037-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPA1 (extracellular)-FITC Antibody

catalog :

ACC-037-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Wu C, Wu C, Su T, Kou Y, Kor C, Lee T, et al. Renal Tubular TRPA1 as a Risk Factor for Recovery of Renal Function from Acute Tubular Necrosis. J Clin Med. 2019;8: pubmed publisher

image

image 1 :

Cell surface detection of TRPA1 by direct flow cytometry in live intact mouse J774 macrophage cells: Cell surface detection of TRPA1 by direct flow cytometry in live intact mouse J774 macrophage cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-TRPA1 (extracellular)-FITC Antibody (#ACC-037-F), (2.5µg).

product information

CAT :

ACC-037-F

SKU :

ACC-037-F_15 mcl

Product Name :

Anti-TRPA1 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O75762

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC037

Homology :

Bovine - identical; Dog - 13/14 amino acid residues identical; Rat - 12/14 amino acid residues identical; Mouse - 9/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

1st extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)NSTGIINETSDHSE, corresponding to amino acid residues 747-760 of human TRPA1

Product Page - Scientific background :

The TRPA family is comprised of only one mammalian member, the TRPA1 (formerly named ANKTM1). TRPA1 is expressed in peripheral sensory neurons, where it is suggested to contribute to the detection of painful stimuli.1Originally, it was thought that TRPA channels sensed painfully cold temperatures,2 but a more conservative description is that TRPA1 is sensitive to membrane/cytoskeletal perturbations caused by low temperatures3-5 and perhaps stretch.6 In addition, it is sensitive to pungent natural compounds present in cinnamon oil, mustard oil, and wintergreen oil.TRPA1 is also expressed in hair cells, where its role in sensing mechanical forces is still unclear and controversial.1TRPA1 has a similar structure to all other TRP ion channels; six transmembrane domains, intracellular N-and C-terminus. However, the N-terminal domain possesses 17 ankyrin repeats that might indicate its potential role as a mechanosensor.6,7In addition, TRPA1 is expressed in nociceptive neurons expressing TRPV1 and might serve as a marker for polymodal nociceptors.8

Applications may also work in :

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily A member 1, ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120, Wasabi Receptor

Short Description :

A Rabbit Polyclonal Antibody to TRPA1 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-TRPA1 (extracellular) Antibody (#ACC-037) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry and live cell imaging applications. It has been designed to recognize TRPA1 from mouse, rat, and human samples. Anti-TRPA1 (extracellular)-FITC Antibody (#ACC-037-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily A member 1, ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120, Wasabi Receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPA1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC FC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-037), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage