Anti-TRPV6 Antibody | Alomone Labs ACC-036 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPV6 Antibody

catalog :

ACC-036

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 10

Reference
Kim D, Kim S, Yang E. RNA interference mediated suppression of TRPV6 inhibits the progression of prostate cancer in vitro by modulating cathepsin B and MMP9 expression. Investig Clin Urol. 2021;62:447-454 pubmed publisher
Bobkov D, Yudintceva N, Lomert E, Shatrova A, Kever L, Semenova S. Lipid raft integrity is required for human leukemia Jurkat T-cell migratory activity. Biochim Biophys Acta Mol Cell Biol Lipids. 2021;1866:158917 pubmed publisher
Crowley L, Cambuli F, Aparicio L, Shibata M, Robinson B, Xuan S, et al. A single-cell atlas of the mouse and human prostate reveals heterogeneity and conservation of epithelial progenitors. elife. 2020;9: pubmed publisher
Park S, Yoo Y, Jung E, Jeung E. The effect of steroid hormone on the expression of the calcium-processing proteins in the immature female rat brain. J Chem Neuroanat. 2020;105:101767 pubmed publisher
Jeon B, Yoo Y, Jung E, Jeung E. Dexamethasone Treatment Increases the Intracellular Calcium Level Through TRPV6 in A549 Cells. Int J Mol Sci. 2020;21: pubmed publisher
Anand P, Filipenko P, Huaman J, Lyudmer M, Hossain M, Santamaría C, et al. Selective Inhibition of Liver Cancer Cells Using Venom Peptide. Mar Drugs. 2019;17: pubmed publisher
Bächinger D, Egli H, Goosmann M, Monge Naldi A, Eckhard A. Immunolocalization of calcium sensing and transport proteins in the murine endolymphatic sac indicates calciostatic functions within the inner ear. Cell Tissue Res. 2019;378:163-173 pubmed publisher
Tran D, Jung E, Ahn C, Lee J, Yoo Y, Jeung E. Effects of Bisphenol A and 4-tert-Octylphenol on Embryo Implantation Failure in Mouse. Int J Environ Res Public Health. 2018;15: pubmed publisher
Suzuki Y, Chitayat D, Sawada H, Deardorff M, McLaughlin H, Begtrup A, et al. TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism. Am J Hum Genet. 2018;102:1104-1114 pubmed publisher
Gao D, Zhang B, Leung M, Au S, Wong P, Shum W. Coupling of TRPV6 and TMEM16A in epithelial principal cells of the rat epididymis. J Gen Physiol. 2016;148:161-82 pubmed publisher

image

image 1 :

Western blot analysis of Human PC-3 prostate cancer cell lysate: - 1. Anti-TRPV6 Antibody (#ACC-036), (1:200).2. Anti-TRPV6 Antibody, preincubated with TRPV6 Blocking Peptide (#BLP-CC036).

image 2 :

Expression of TRPV6 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPV6 Antibody (#ACC-036), (1:100). A. TRPV6 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV6 and Parvalbumin demonstrates colocalization (arrows).

image 3 :

Western blot analysisof PC-3 cell lysate: - 1.Anti-TRPV6Antibody (#ACC-036) (1:200).2. Anti-TRPV6 Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-036

SKU :

ACC-036-CF_0.2 ml

Product Name :

Anti-TRPV6 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H1D0

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC036

Homology :

Rat, mouse - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)NRGLEDGESWEYQI, corresponding to amino acid residues 712-725 of human TRPV6

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV6 (CaT1) and TRPV5 (ECaC) are unique members of the TRP family. Both are epithelial calcium channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV6 and TRPV5 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption. TRPV6 is highly expressed in the placenta, small intestine and kidney.6,7While not being expressed in benign prostate tissue, TRPV6 was found to be upregulated in prostate cancer tissue and correlation between expression and tumor grade was shown. Finally, TRPV6 was suggested as prognostic marker and as a promising candidate for therapeutic strategies.8,9

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6

Short Description :

A Rabbit Polyclonal Antibody to TRPV6 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human TRPV6. Anti-TRPV6 Antibody (#ACC-036) can be used in western blot, immunoprecipitation, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize TRPV6 from human, rat, and mouse samples.

Negative Control :

BLP-CC036

Positive Control :

Synonyms :

Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage