Anti-TRPV5 Antibody | Alomone Labs ACC-035 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPV5 Antibody

catalog :

ACC-035

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Park S, Yoo Y, Jung E, Jeung E. The effect of steroid hormone on the expression of the calcium-processing proteins in the immature female rat brain. J Chem Neuroanat. 2020;105:101767 pubmed publisher
Ferdaus M, Gratreak B, Miller L, Si J, McCormick J, Yang C, et al. WNK4 limits distal calcium losses following acute furosemide treatment. Physiol Rep. 2019;7:e14195 pubmed publisher
Bächinger D, Egli H, Goosmann M, Monge Naldi A, Eckhard A. Immunolocalization of calcium sensing and transport proteins in the murine endolymphatic sac indicates calciostatic functions within the inner ear. Cell Tissue Res. 2019;378:163-173 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 3) and human PC3 prostate cancer cell line (lanes 2 and 4) lysates: - 1,2. Anti-TRPV5 Antibody (#ACC-035), (1:200).3,4. Anti-TRPV5 Antibody, preincubated with TRPV5 Blocking Peptide (#BLP-CC035).

image 2 :

Expression of TRPV5 in rat kidney - Immunohistochemical staining of rat kidney paraffin embedded section using Anti-TRPV5 Antibody (#ACC-035), (1:100). TRPV5 is expressed in collecting ducts (CD) in the renal cortex. Note that both proximal tubules (PT) and distal tubules (DT) are negative. Hematoxilin is used as the counterstain.

image 3 :

Expression of TRPV5 in rat kidney - Immunohistochemical staining of rat kidney paraffin embedded section usingAnti-TRPV5Antibody (#ACC-035) (1:100). TRPV5 is expressed in collecting ducts (CD) in the renal cortex. Note that both proximal tubules (PT) and distal tubules (DT) are negative. Hematoxilin is used as the counterstain.

product information

CAT :

ACC-035

SKU :

ACC-035-CF_0.2 ml

Product Name :

Anti-TRPV5 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8NDW5

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC035

Homology :

Rat - 11/15 amino acid residues identical; rabbit - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)GLNLSEGDGEEVYHF, corresponding to amino acid residues 715-729 of human TRPV5

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR, the TRP family is comprised of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV5 (ECaC) and TRPV6 (CaT1) are unique members of the TRP family. Both are epithelial Ca2+ channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N- and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV5 and TRPV6 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption.6,7 TRPV5 is preferentially expressed in the kidney.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, CaT2, Epithelial calcium channel 1, ECaC1, OTRPC3

Short Description :

A Rabbit Polyclonal Antibody to TRPV5 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human TRPV5. Anti-TRPV5 Antibody (#ACC-035) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize TRPV5 from human, mouse and rat samples.

Negative Control :

BLP-CC035

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, CaT2, Epithelial calcium channel 1, ECaC1, OTRPC3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage