Anti-TRPV4-ATTO Fluor-550 Antibody | Alomone Labs ACC-034-AO product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPV4-ATTO Fluor-550 Antibody

catalog :

ACC-034-AO

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Willard V, Leddy H, Palmer D, Wu C, Liedtke W, Guilak F. Transient receptor potential vanilloid 4 as a regulator of induced pluripotent stem cell chondrogenesis. Stem Cells. 2021;39:1447-1456 pubmed publisher
Tomilin V, Pyrshev K, Khayyat N, Zaika O, Pochynyuk O. With-No-Lysine Kinase 1 (WNK1) Augments TRPV4 Function in the Aldosterone-Sensitive Distal Nephron. Cells. 2021;10: pubmed publisher
Wang H, Zhang B, Wang X, Mao J, Li W, Sun Y, et al. TRPV4 Overexpression Promotes Metastasis Through Epithelial-Mesenchymal Transition in Gastric Cancer and Correlates with Poor Prognosis. Onco Targets Ther. 2020;13:8383-8394 pubmed publisher
Scheraga R, Abraham S, Grove L, Southern B, Crish J, Perelas A, et al. TRPV4 Protects the Lung from Bacterial Pneumonia via MAPK Molecular Pathway Switching. J Immunol. 2020;204:1310-1321 pubmed publisher
Heppner T, Hennig G, Nelson M, Vizzard M. Rhythmic Calcium Events in the Lamina Propria Network of the Urinary Bladder of Rat Pups. Front Syst Neurosci. 2017;11:87 pubmed publisher

image

image 1 :

Expression of TRPV4 in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections with Anti-TRPV4-ATTO Fluor-550 Antibody (#ACC-034-AO), (1:20). TRPV4 is present in DRG neurons (red). Note that staining is stronger in small cells (arrows). Hoechst 33342 is used as the counterstain.

product information

CAT :

ACC-034-AO

SKU :

ACC-034-AO_50 mcl

Product Name :

Anti-TRPV4-ATTO Fluor-550 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9ERZ8

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC034

Homology :

Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.Alomone Labs offers a highly specific antibody directed against an intracellular epitope of TRPV4. Anti-TRPV4 Antibody (#ACC-034) can be used in western blot, immunocytochemical, and immunohistochemical applications and recognizes TRPV4 from rat samples.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

OTRPC4, VRL-2, VR-OAC, TRP12

Short Description :

A Rabbit Polyclonal Antibody to TRPV4 Channel Conjugated to the Fluorescent Dye ATTO-550

Long Description :

Anti-TRPV4 Antibody (#ACC-034) is a highly specific antibody directed against an intracellular epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples. Anti-TRPV4-ATTO Fluor-550 Antibody (#ACC-034-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-TRPV4-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AO

Positive Control :

Synonyms :

OTRPC4, VRL-2, VR-OAC, TRP12

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-034), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:20

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage