Anti-TRPV3 (extracellular)-ATTO Fluor-633 Antibody | Alomone Labs ACC-033-FR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPV3 (extracellular)-ATTO Fluor-633 Antibody

catalog :

ACC-033-FR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Zhao J, Munanairi A, Liu X, Zhang J, Hu L, Hu M, et al. PAR2 Mediates Itch via TRPV3 Signaling in Keratinocytes. J Invest Dermatol. 2020;140:1524-1532 pubmed publisher

image

image 1 :

Expression of TRPV3 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPV3 (extracellular)-ATTO Fluor-633 Antibody (#ACC-033-FR). A. TRPV3 staining (purple) is detected in DRG neurons. B. Nuclear staining using DAPI as the counterstain (Blue). C. Merge images of A and B.

image 2 :

Expression of TRPV3 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) usingAnti-TRPV3 (extracellular)-ATTO-633Antibody (#ACC-033-FR). A. TRPV3 staining (purple) is detected in DRG neurons. B. Nuclear staining using DAPI as the counterstain (Blue). C. Merge images of A and B.

product information

CAT :

ACC-033-FR

SKU :

ACC-033-FR_50 mcl

Product Name :

Anti-TRPV3 (extracellular)-ATTO Fluor-633 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8NET8

Applications :

FC IC IF IHC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC033

Homology :

Rat - 13/15 amino acid residues identical; mouse - 12/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

1st extracellular loop

Label :

ATTO-633. Maximum absorption 629 nm; maximum fluorescence 657 nm. The fluorescence is excited most efficiently in the 610 - 645 nm range. This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)REEEAIPHPLALTHK, corresponding to amino acid residues 464-478 of human TRPV3

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3According to IUPHAR, the TRP family is composed of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (52°C).6,7TRPV3 is highly expressed in skin keratinocytes. It forms a nonselective cation channel that is activated by hot temperatures in the innocuous and noxious range. The channel is also expressed in oral and nasal cavities where it has been postulated to play a role in flavor sensation. TRPV3 is also implicated as a molecular target of some allergens and skin sensitizers.8

Applications may also work in :

FC IC IF IHC LCI

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily V member 3, Vanilloid receptor-like 3, VRL3

Short Description :

A Rabbit Polyclonal Antibody to TRPV3 Channel Conjugated to the Fluorescent Dye ATTO-633

Long Description :

Anti-TRPV3 (extracellular) Antibody (#ACC-033) is a highly specific antibody directed against an extracellular epitope of human TRPV3. The antibody can be used for western blot, immunohistochemical and immunocytochemical applications. As the antibody recognizes an extracellular epitope, it can be used as a tool to detect TRPV3 in live cells. The antibody has been designed to recognize TRPV3 from human, rat and mouse samples. Anti-TRPV3 (extracellular)-ATTO Fluor-633 Antibody (#ACC-033-FR) is directly labeled with an ATTO-633 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Anti-TRPV3 (extracellular)-ATTO Fluor-633 Antibody has been tested in immunohistochemical staining and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-CC033

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily V member 3, Vanilloid receptor-like 3, VRL3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV3

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-033), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage