Anti-TRPV3 (extracellular)-Biotin Antibody | Alomone Labs ACC-033-B product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPV3 (extracellular)-Biotin Antibody

catalog :

ACC-033-B

clonality :

polyclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Zhao J, Munanairi A, Liu X, Zhang J, Hu L, Hu M, et al. PAR2 Mediates Itch via TRPV3 Signaling in Keratinocytes. J Invest Dermatol. 2020;140:1524-1532 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-TRPV3 (extracellular)-Biotin Antibody (#ACC-033-B), (1:5000)2. Anti-TRPV3 (extracellular)-Biotin Antibody, preincubated with the control antigen.

image 2 :

Expression of TRPV3 in rat PC12 cells - Cell surface detection ofTRPV3 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells usingAnti-TRPV3 (extracellular)-BiotinAntibody (#ACC-033-B) (1:50) (red). B. Merge of A with the live view of the cell.

image 3 :

Western blot analysis of rat cerebellum lysate (lanes 1 and 3) and rat heart membrane (lanes 2 and 4): - 12. Anti-Rat CMKLR1 (extracellular) antibody (1:200) (ACR-033).34. Anti-Rat CMKLR1 (extracellular) antibody preincubated with the negative control antigen.

product information

CAT :

ACC-033-B

SKU :

ACC-033-B_50 mcl

Product Name :

Anti-TRPV3 (extracellular)-Biotin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8NET8

Applications :

IC IF LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC033

Homology :

Rat - 13/15 amino acid residues identical; mouse - 12/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

1st extracellular loop

Label :

Biotin

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)REEEAIPHPLALTHK, corresponding to amino acid residues 464-478 of human TRPV3

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3According to IUPHAR, the TRP family is composed of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (52°C).6,7TRPV3 is highly expressed in skin keratinocytes. It forms a nonselective cation channel that is activated by hot temperatures in the innocuous and noxious range. The channel is also expressed in oral and nasal cavities where it has been postulated to play a role in flavor sensation. TRPV3 is also implicated as a molecular target of some allergens and skin sensitizers.8

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily V member 3, Vanilloid receptor-like 3, VRL3

Short Description :

A Rabbit Polyclonal Antibody to TRPV3 Channel Conjugated to Biotin

Long Description :

Anti-TRPV3 (extracellular) Antibody (#ACC-033) is a highly specific antibody directed against an extracellular epitope of human TRPV3. The antibody can be used in western blot, immunohistochemical and immunocytochemical applications. As the antibody recognizes an extracellular epitope, it can be used as a tool to detect TRPV3 in live cells. The antibody has been designed to recognize TRPV3 from human, rat and mouse samples. Anti-TRPV3 (extracellular)-Biotin Antibody (#ACC-033-B) is directly labeled with biotin. Strepavidin tagged with HRP or with a fluorescent probe can then be used to detect the protein. The biotin/strepavidin system is ideal for minimizing cross-reactivity when same species antibodies are simultaneously used. Anti-TRPV3 (extracellular)-Biotin Antibody has been tested in western blot and live cell imaging and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-CC033

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily V member 3, Vanilloid receptor-like 3, VRL3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV3

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:5000

more info or order :

Alomone Labs product webpage